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Products:Neuroscience >> Neurology process >> Neural Signal Transduction
Overview
Product name
Anti-PAK3 (phospho S154) antibody
See all PAK3 products (6) ...
Description
Rabbit polyclonal to PAK3 (phospho S154)
Specificity
ab80181 detects endogenous levels of PAK3 only when phosphorylated at serine 154.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse
Immunogen
Synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of serine 154 (Y-M-SP-F-T).
Positive control
Human brain tissue; rat heart cell extracts.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab80181 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Neuroscience >> Neurology process >> Neural Signal Transduction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 62 kDa.
ELISA
ELISA: 1/20,000ELISA: 1/20,000
IHC-P: 1/50 - 1/100.
Target
Relevance
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation.
Cellular localization
Cytoplasmic
Alternative names
- Beta PAK antibody
- bPAK antibody
- CDKN1A antibody
see all
Database links
- Entrez Gene: 5063 Human
- Entrez Gene: 18481 Mouse
- Entrez Gene: 29433 Rat
- Omim: 300142 Human
- SwissProt: O75914 Human
- SwissProt: Q17RE5 Human
- SwissProt: Q61036 Mouse
- SwissProt: Q62829 Rat
see all
Anti-PAK3 (phospho S154) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK3 (phospho S154) antibody (ab80181)
ab80181, at a 1/50 dilution, staining PAK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.
Western blot - PAK3 (phospho S154) antibody (ab80181)
All lanes : Anti-PAK3 (phospho S154) antibody (ab80181) at 1/500 dilution
Lane 1 : rat heart cell extracts
Lane 2 : rat heart cell extracts with immunizing phospho peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size : 62 kDa
Observed band size : 62 kDa
References for Anti-PAK3 (phospho S154) antibody (ab80181)
ab80181 has not yet been referenced specifically in any publications.
Publishing research using ab80181? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"