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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PAK4 antibody (ab62509)
Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml
Lane 1 : SW480 cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 64 kDa
Observed band size : 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK4 antibody (ab62509)
Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
Immunocytochemistry/ Immunofluorescence-PAK4 antibody(ab62509)
ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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