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Products:Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Overview
Product name
Anti-PAK4 antibody
See all PAK4 products (6) ...
Description
Rabbit polyclonal to PAK4
Specificity
This antibody reacts with PAK4
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
A 13 amino acid synthetic peptide from near the center of human PAK4
Positive control
SW480 cell lysate and human colon tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Kinases/phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 - 2 µg/ml.Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P: Use a concentration of 10 µg/ml
Target
Function
Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Tissue specificity
Highest expression in prostate, testis and colon.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on serine residues when activated by CDC42/p21.
Phosphorylated on tyrosine residues upon stimulation of FGFR2.
Target information above from: UniProt accessionO96013
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- KIAA1142 antibody
- p21 activated kinase 4 antibody
- p21(CDKN1A) activated kinase 4 antibody
see all
Database links
- Entrez Gene: 10298 Human
- Omim: 605451 Human
- SwissProt: O96013 Human
- Unigene: 20447 Human
Anti-PAK4 antibody images:
Western blot - PAK4 antibody (ab62509)
Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml
Lane 1 : SW480 cell lysate
Lane 2 : SW480 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 64 kDa
Observed band size : 64 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK4 antibody (ab62509)
Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
Immunocytochemistry/ Immunofluorescence-PAK4 antibody(ab62509)
ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PAK4 antibody (ab62509)
ab62509 has not yet been referenced specifically in any publications.
Publishing research using ab62509? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"