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PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)

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    Overview

    Product name

    PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate

    General notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

    Properties

    Form

    Liquid

    Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9

    Concentration

    Concentration information loading...

    Research areas

    Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other

    Background

    PANC-1 cells are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. The cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth-factor receptor 2 (HER2/neu) oncogene (which is present in 60-70% of human pancreatic carcinomas) but are estrogen receptor (ER) negative. Cells are also negative for MUC4 (Sialomucin complex SMC, an intramembrane ligand for ErbB2) and positive for Smad4 (a TGF beta signalling cascade protein inactivated in human gastrointestinal cancers).

    Applications

    Application notes

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)

    ab14882 has not yet been referenced specifically in any publications.

    Publishing research using ab14882? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"