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Product Name
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PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate
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Product type
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Lysates
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Description
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PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate
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Application notes
(see key)
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Recommended dilutions
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Kits/ Lysates/ Other >> Lysates >> Nuclear Lysates >> Human >> Other
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Relevance
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PANC-1 cells are used as an in vitro model of non-endocrine pancreatic cancer for tumorigenicity studies. The cells possess the type B phenotype for glucose-6-phosphate dehydrogenase G6PD and overexpress heregulin/human epidermal growth-factor receptor 2 (HER2/neu) oncogene (which is present in 60-70% of human pancreatic carcinomas) but are estrogen receptor (ER) negative. Cells are also negative for MUC4 (Sialomucin complex SMC, an intramembrane ligand for ErbB2) and positive for Smad4 (a TGF beta signalling cascade protein inactivated in human gastrointestinal cancers).
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Storage buffer
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Preservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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Form
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Liquid
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Concentration
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7.110 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PANC-1
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