Anti-PAPP A antibody [9] (ab52030)
Key features and details
- Mouse monoclonal [9] to PAPP A
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-PAPP A antibody [9]
See all PAPP A primary antibodies -
Description
Mouse monoclonal [9] to PAPP A -
Host species
Mouse -
Specificity
This antibody is specific for native human PAPP A. Heat and SDS denatured PAPP A is also recognized, but not acid treated PAPP A. -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) (Human)
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Epitope
The epitope is located on the PAPP A subunit of the heterotetramer. -
General notes
Clone number also known as 004-09.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 2.9% Sodium chloride, 0.0268% PBS -
Concentration information loading...
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Purification notes
Protein A/G purified -
Clonality
Monoclonal -
Clone number
9 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. -
Tissue specificity
High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. -
Sequence similarities
Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains. -
Developmental stage
Present in serum and placenta during pregnancy; levels increase throughout pregnancy. -
Post-translational
modificationsThere appear to be no free sulfhydryl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 5069 Human
- Omim: 176385 Human
- SwissProt: Q13219 Human
- Unigene: 643599 Human
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Alternative names
- ASBABP2 antibody
- Aspecific BCL2 ARE binding protein 2 antibody
- Differentially placenta 1 expressed protein antibody
see all
Images
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Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using frozen tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.
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Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using parrafin embedded tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab52030 has been referenced in 3 publications.
- Ortega MA et al. Abnormal proinflammatory and stressor environmental with increased the regulatory cellular IGF-1/PAPP-A/STC and Wnt-1/β-Catenin canonical pathway in placenta of women with Chronic venous Disease during Pregnancy. Int J Med Sci 18:2814-2827 (2021). PubMed: 34220309
- Ortega MA et al. Chronic Venous Disease Patients Showed Altered Expression of IGF-1/PAPP-A/STC-2 Axis in the Vein Wall. Biomed Res Int 2020:6782659 (2020). PubMed: 33381575
- Sun Y et al. MiR-490-3p modulates the proliferation of vascular smooth muscle cells induced by ox-LDL through targeting PAPP-A. Cardiovasc Res 100:272-9 (2013). PubMed: 23821525