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Anti-PAPP A antibody [004-09] (ab52030)

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Overview

Product name

Anti-PAPP A antibody [004-09]
See all PAPP A products (17) ...

Description

Mouse monoclonal [004-09] to PAPP A

Specificity

This antibody is specific for native human PAPP A. Heat and SDS denatured PAPP A is also recognized, but not acid treated PAPP A.

Tested applications

WB, ELISA, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Full length native protein (purified) (Human)

Epitope

The epitope is located on the PAPP A subunit of the heterotetramer.

Properties

Form

Liquid

Storage instructions

Store at +4°C in the dark.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4

Concentration

Concentration information loading...

Purification notes

Protein A/G purified

Clonality

Monoclonal

Clone number

004-09

Isotype

IgG2b

Light chain type

kappa

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Serum Proteins

  • Immunohistochemistry (Frozen sections) - PAPP A antibody [004-09] (ab52030)Immunohistochemistry (Frozen sections) - PAPP A antibody [004-09] (ab52030) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - PAPP A antibody [004-09] (ab52030)Immunohistochemistry (Paraffin-embedded sections) - PAPP A antibody [004-09] (ab52030) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab52030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide

    WB

     WB: Use at an assay depe...Read more →

    WB: Use at an assay dependent dilution. Predicted molecular weight: 181 kDa.

  • ShowHide

    ELISA

     ELISA: 1/8000(This antib...Read more →

    ELISA: 1/8000(This antibody can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP A in coat.)

  • ShowHide1 Image

    IHC-P

     IHC-P: Use at an assay d...Read more →

    IHC-P: Use at an assay dependent dilution.

  • ShowHide1 Image

    IHC-Fr

     IHC-Fr: Use at an assay ...Read more →

    IHC-Fr: Use at an assay dependent dilution.

Target

Function

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Tissue specificity

High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.

Sequence similarities

Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains.

Developmental stage

Present in serum and placenta during pregnancy; levels increase throughout pregnancy.

Post-translational
modifications

There appear to be no free sulfhydryl groups.

Cellular localization

Secreted.

Target information above from: UniProt accessionQ13219 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ASBABP2 antibody
  • Aspecific BCL2 ARE binding protein 2 antibody
  • Differentially placenta 1 expressed protein antibody
  • DIPLA1 antibody
  • EC 3.4.24.79 antibody
  • IGF dependent IGFBP 4 protease antibody
  • IGF-dependent IGFBP-4 protease antibody
  • IGFBP 4ase antibody
  • IGFBP-4ase antibody
  • IGFBP4ase antibody
  • Insulin like growth factor dependent IGF binding protein 4 protease antibody
  • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
  • PAPA antibody
  • PAPP A antibody
  • PAPP-A antibody
  • PAPP1_HUMAN antibody
  • PAPPA antibody
  • PAPPA1 antibody
  • Pappalysin 1 precursor antibody
  • Pappalysin-1 antibody
  • Pregnancy Associated Plasma Protein A antibody
  • Pregnancy associated plasma protein A pappalysin 1 antibody
  • Pregnancy-associated plasma protein A antibody
see all

Database links

Anti-PAPP A antibody [004-09] images:

  Immunohistochemistry (Frozen sections) - PAPP A antibody [004-09] (ab52030)

Immunohistochemistry (Frozen sections) - PAPP A antibody [004-09] (ab52030)

Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using frozen tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.

  Immunohistochemistry (Paraffin-embedded sections) - PAPP A antibody [004-09] (ab52030)

Immunohistochemistry (Paraffin-embedded sections) - PAPP A antibody [004-09] (ab52030)

Ab52030 staining human PAPP A in human placenta tissue by immunohistochemistry using parrafin embedded tissue. Staining (red-brown) is restricted to the syncytiotrophoblast of the chorionic villi.

Protocols

  • Recommended IHC protocol with ab52030

    • References for Anti-PAPP A antibody [004-09] (ab52030)

    ab52030 has not yet been referenced specifically in any publications.

    Publishing research using ab52030? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"