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Product Name
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PAPP A antibody [10A5]
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See all PAPP A antibodies (16)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [10A5] to PAPP A
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Immunogen
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Purified human PAPPA antigen.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB, sELISA
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
sELISA: Can be used in immunoassay for the detection of human PAPPA antigen as an antibody pair with Mouse monoclonal [3C8] to PAPPA (ab10194) for coating.Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 500 kDa. Recognizes heterotetrameric complex in Western Blotting after SDS-electrophoresis in non-reducing conditions.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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PAPP A was originally isolated from pregnancy serum as heterotetrameric protein complex with molecular weight approximately 500 kDa, consisting of two PAPP A subunits disulfide-linked with two subunits of the proform of eosinophil major basic protein (proMBP). In such a complex proMBP functions as an inhibitor of PAPP A proteinase activity. PAPP A is widely used as a marker of some pathologies during pregnancy. Reduced level of PAPP A in pregnancy blood in the first trimester is associated with fetal Down syndrome. PAPP A is considered as one of the best biochemical markers in early pregnancy used to screen for Down syndrome during the first trimester (Wald, N. J. et al).
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PAPP A antibody [10A5] (ab10193) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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10A5
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Myeloma
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Sp2/0
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified by chromatography on protein A sepharose. Purity tested by electrophoresis.
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Notes
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Concentration varies from lot to lot and can be provided on request.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAPP A antibody [10A5] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PAPP A
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PAPP A antibody [10A5] - more information
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