You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-PAPP A antibody [18A10] (ab10196)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-PAPP A antibody [18A10]
See all PAPP A products (17) ...

Description

Mouse monoclonal [18A10] to PAPP A

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Purified human PAPPA antigen

General notes

Concentration varies from lot to lot and can be provided on request.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein A purified

Purification notes

Purified by chromatography on protein A. Purity tested by electrophoresis

Clonality

Monoclonal

Clone number

18A10

Myeloma

Sp2/0

Isotype

IgG2a

Research areas

Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Serum Proteins

  • Western blot - PAPP A antibody [18A10] (ab10196)Western blot - PAPP A antibody [18A10] (ab10196) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10196 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 200 kDa under reducing conditions. (predicted molecular weight: 195 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.

Tissue specificity

High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.

Sequence similarities

Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains.

Developmental stage

Present in serum and placenta during pregnancy; levels increase throughout pregnancy.

Post-translational
modifications

There appear to be no free sulfhydryl groups.

Cellular localization

Secreted.

Target information above from: UniProt accessionQ13219 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ASBABP2 antibody
  • Aspecific BCL2 ARE binding protein 2 antibody
  • Differentially placenta 1 expressed protein antibody
  • DIPLA1 antibody
  • EC 3.4.24.79 antibody
  • IGF dependent IGFBP 4 protease antibody
  • IGF-dependent IGFBP-4 protease antibody
  • IGFBP 4ase antibody
  • IGFBP-4ase antibody
  • IGFBP4ase antibody
  • Insulin like growth factor dependent IGF binding protein 4 protease antibody
  • Insulin-like growth factor-dependent IGF-binding protein 4 protease antibody
  • PAPA antibody
  • PAPP A antibody
  • PAPP-A antibody
  • PAPP1_HUMAN antibody
  • PAPPA antibody
  • PAPPA1 antibody
  • Pappalysin 1 precursor antibody
  • Pappalysin-1 antibody
  • Pregnancy Associated Plasma Protein A antibody
  • Pregnancy associated plasma protein A pappalysin 1 antibody
  • Pregnancy-associated plasma protein A antibody
see all

Database links

Anti-PAPP A antibody [18A10] images:

  Western blot - PAPP A antibody [18A10] (ab10196)

Western blot - PAPP A antibody [18A10] (ab10196)



Predicted band size : 195 kDa


Detection of human PAPP-A by ab10196 in Western Blotting. A. ab10196 recognizes PAPP-A subunit after SDS-gel electrophoresis in reducing conditions (gradient gel 5 % – 15 %) B. ab10196 recognizes heterotetrameric complex (gradient gel 2.5 % – 10 %)

References for Anti-PAPP A antibody [18A10] (ab10196)

This product has been referenced in:

  • Escamilla-Gómez Vet al. Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens Bioelectron 24:2678-83 (2009). Human.Read more (PubMed: 19261459) »

See 1 publication for this product

Publishing research using ab10196? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"