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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-PAPP A antibody
See all PAPP A products (17) ...
Description
Rabbit polyclonal to PAPP A
Specificity
ab59088 recognizes all of the published forms of PAPP A. We have a range of domain specific antibodies for this target. For a full list please see all PAPP A antibodies.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
A synthetic peptide based on the LamGL domain of human PAPP A.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Other Antibodies >> Other Antibodies
Cardiovascular >> Blood >> Serum Proteins
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Western blot - PAPP A antibody (ab59088)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000 - 1/5000 dependent on substrate. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Predicted molecular weight: 181 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF.
Tissue specificity
High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts.
Sequence similarities
Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains.
Developmental stage
Present in serum and placenta during pregnancy; levels increase throughout pregnancy.
Post-translational
modifications
There appear to be no free sulfhydryl groups.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ13219
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ASBABP2 antibody
- Aspecific BCL2 ARE binding protein 2 antibody
- Differentially placenta 1 expressed protein antibody
see all
Database links
- Entrez Gene: 5069 Human
- Omim: 176385 Human
- SwissProt: Q13219 Human
- Unigene: 643599 Human
- Unigene: 728832 Human
Anti-PAPP A antibody images:
Western blot - PAPP A antibody (ab59088)
All lanes : Anti-PAPP A antibody (ab59088) at 1/1000 dilution
Lane 1 : Recombinant Human Pappalysin-1 at 0.04 µg
Lane 2 : Recombinant Human Pappalysin-1 at 0.02 µg
Lane 3 : Recombinant Human Pappalysin-1 at 0.01 µg
Lane 4 : Recombinant Human Pappalysin-1 at 0.005 µg
Lane 5 : Recombinant Human Pappalysin-2 at 0.04 µg
Lane 6 : Recombinant Human Pappalysin-2 at 0.02 µg
Lane 7 : Recombinant Human Pappalysin-2 at 0.01 µg
Lane 8 : Recombinant Human Pappalysin-2 at 0.005 µg
Predicted band size : 181 kDa
References for Anti-PAPP A antibody (ab59088)
ab59088 has not yet been referenced specifically in any publications.
Publishing research using ab59088? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"