Anti-PAR2 antibody (ab124227)
Key features and details
- Rabbit polyclonal to PAR2
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-PAR2 antibody
See all PAR2 primary antibodies -
Description
Rabbit polyclonal to PAR2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide within Human PAR2 aa 300-370 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary. The immunogen is directed against a cytoplasmic region of PAR2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Contains antibody stabilization buffer. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab123227 is purified on an antigen-based affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/100.
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Notes |
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IHC-P
1/100. |
Target
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Function
Receptor for trypsin and trypsin-like enzymes coupled to G proteins that stimulate phosphoinositide hydrolysis. May have a role in the regulation of vascular tone. -
Tissue specificity
Widely expressed in tissues with especially high levels in pancreas, liver, kidney, small intestine, and colon. Moderate expression is detected in many organs, but none in brain or skeletal muscle. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsA proteolytic cleavage generates a new N-terminus that functions as a tethered ligand. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 116677 Rat
- SwissProt: Q63645 Rat
- Unigene: 10543 Rat
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Alternative names
- Coagulation factor II receptor like 1 antibody
- Coagulation factor II receptor-like 1 antibody
- Coagulation factor II thrombin receptor like 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling PAR2 with ab124227 at 1/100 dilution. Counterstained with hematoxylin. 40X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling PAR2 with ab124227 at 1/100 dilution. Counterstained with hematoxylin. 5X magnification.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab124227 has been referenced in 3 publications.
- Huuskonen MT et al. Protection of ischemic white matter and oligodendrocytes in mice by 3K3A-activated protein C. J Exp Med 219:N/A (2022). PubMed: 34846535
- Sokolina K et al. Systematic protein-protein interaction mapping for clinically relevant human GPCRs. Mol Syst Biol 13:918 (2017). IP . PubMed: 28298427
- Li M et al. Activation of protease-activated receptor-2 is associated with increased expression of inflammatory factors in the adipose tissues of obese mice. Mol Med Rep 12:6227-34 (2015). PubMed: 26252901