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Immunoprecipitation - PAR6 antibody (ab45394)
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Product Name
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PAR6 antibody
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See all PAR6 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAR6
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Immunogen
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Synthetic peptide derived from within the CRIB loop (Cdc42/Rac1 interaction binding domain in the mid-region of the protein) of dog Par6b.
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Reacts with
(species key)
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Hu, Ms, Dog
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Specificity
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Recognises all three par6 isoforms (a, b and c)
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Tested applications
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ICC/IF, IP, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: 1/300.
WB: 1/1000. Predicted molecular weight: 37 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane
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Research areas
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Stem Cells >> Cancer Stem Cells >> Intracellular
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
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Relevance
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PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Whole antiserum
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAR6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAR6 antibody images, references, products related to ab45394 and other tools.
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PAR6 antibody images:
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 Immunoprecipitation - PAR6 antibody (ab45394)
293T cells were transiently transfected with Par6 isoforms a, b, or c tagged with HA.The proteins were immunoprecipitated with rat anti-HA and then immunoblotted with either rat anti-HA or rabbit anti-Par6.The Par6 antisera recognizes all 3 of the human Par6 isoforms. The canine and mouse isoforms are also recognized (data not shown).
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References for PAR6 antibody (ab45394)
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This product has been used in: (two most recent references)
Yamanaka Y et al. FGF signal-dependent segregation of primitive endoderm and epiblast in the mouse blastocyst. Development 137:715-24 (2010). ICC/IF; Mouse. PubMed: 20147376
Mashukova A et al. Rescue of atypical protein kinase C in epithelia by the cytoskeleton and Hsp70 family chaperones. J Cell Sci 122:2491-503 (2009). WB. PubMed: 19549684
If you publish research using ab45394 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PAR6
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PAR6 antibody - more information
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Customer reviews (feedback) regarding PAR6 antibody |
Customer FAQs regarding PAR6 antibody |
Protocols for PAR6 antibody |
Price and availability of products related to PAR6 antibody |
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PAR6 antibody - related products:
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PAR6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAR6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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