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Products:Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Overview
Product name
Anti-PAR6 antibody
See all PAR6 products (6) ...
Description
Rabbit polyclonal to PAR6
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PAR6.
(Peptide available as ab49775.)
Positive control
This antibody gave a positive signal using a PARD6A partial Human Recombinant Tagged Protein
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Cancer Stem Cells >> Intracellular
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Target
Function
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
Tissue specificity
Expressed in pancreas, skeletal muscle, brain and heart. Weakly expressed in kidney and placenta.
Sequence similarities
Belongs to the PAR6 family.
Contains 1 OPR domain.
Contains 1 PDZ (DHR) domain.
Contains 1 pseudo-CRIB domain.
Domain
The pseudo-CRIB domain together with the PDZ domain is required for the interaction with Rho small GTPases.
The OPR domain mediates interactions with MAP2K5.
The PDZ domain mediates the interaction with CRB3.
Cellular localization
Cytoplasm. Cell membrane. Cell projection > ruffle. Cell junction > tight junction. Colocalizes with GTP-bound CDC42 or RAC1 at membrane ruffles and with PARD3 and PRKCI at epithelial tight junctions.
Target information above from: UniProt accessionQ9NPB6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Par 6 partitioning defective 6 C elegans homolog alpha antibody
- Par 6 partitioning defective 6 homolog alpha C elegans antibody
- PAR 6A antibody
see all
Database links
- Entrez Gene: 50855 Human
- Omim: 607484 Human
- SwissProt: Q9NPB6 Human
- Unigene: 112933 Human
Anti-PAR6 antibody images:
Western blot - Anti-PAR6 antibody (ab49776)
Anti-PAR6 antibody (ab49776) at 1 µg/ml + PARD6A partial Human Recombinant Tagged Protein at 0.2 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 37 kDa
Immunocytochemistry/ Immunofluorescence - PAR6 antibody (ab49776)
ICC/IF image of ab49776 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49776, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells.
References for Anti-PAR6 antibody (ab49776)
This product has been referenced in:
- Ye Pet al. CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biol 10:2 (2009).Read more (PubMed: 19138432) »
- Koch Set al. Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell 20:4816-25 (2009). ICC/IF; Human.Read more (PubMed: 19776352) »
See all 2 publications for this product
Publishing research using ab49776? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"