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Western blot - (ab49776)
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Immunocytochemistry/ Immunofluorescence - PAR6 antibody (ab49776)
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Product Name
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PAR6 antibody
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See all PAR6 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAR6
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human PAR6. (Peptide available as ab49775.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal using a PARD6A partial Human Recombinant Tagged Protein
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Cellular localization
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Cell Membrane
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Research areas
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Stem Cells >> Cancer Stem Cells >> Intracellular
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
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Relevance
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PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAR6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAR6 antibody images, references, products related to ab49776 and other tools.
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PAR6 antibody images:
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 Western blot - (ab49776)
PAR6 antibody (ab49776) at 1 µg/ml + PARD6A partial Human Recombinant Tagged Protein at 0.2 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 37 kDa
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Immunocytochemistry/ Immunofluorescence - PAR6 antibody (ab49776)
ICC/IF image of ab49776 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab49776, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa and HEK 293 cells.
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References for PAR6 antibody (ab49776)
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This product has been used in: (two most recent references)
Ye P et al. CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biol 10:2 (2009). PubMed: 19138432
Koch S et al. Dkk-1 inhibits intestinal epithelial cell migration by attenuating directional polarization of leading edge cells. Mol Biol Cell 20:4816-25 (2009). ICC/IF; Human. PubMed: 19776352
If you publish research using ab49776 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PAR6
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PAR6 antibody - more information
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Customer reviews (feedback) regarding PAR6 antibody |
Customer FAQs regarding PAR6 antibody |
Protocols for PAR6 antibody |
Price and availability of products related to PAR6 antibody |
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PAR6 antibody - related products:
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PAR6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAR6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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