Anti-PAR6 antibody (ab6022)

Status of: #****** : received in late february

#**** : Unfortunately ab45394 is now unpublished. studied species : Mouse application : IF, WB, IP

ANSWER:

Comme convenu, la commande numéro ****** (une unité gratuite de ab6022) a été expédiée aujourd'hui. Vous devriez recevoir le produit demain.

J'espère que l'anti-PAR6 ab6022 fonctionnera chez la souris. Nous serions ravis si vous pouviez nous envoyer une Abreview avec votre feedback sur l'utilisation de ab6022 chez la souris. Le lien pour soumettre cette Abreview est : http://www.abcam.com/PAR6-antibody-ab6022-reviews.html

N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

We have injected the homogenized tumor tissue isolated from human lung into the armpit of mice. After certain time the neoplasm is formed in the armpit. Now we want to know the nature of the neoplasm that is originally from human lung tumor or is a new tumor from mouse. We would like to check the nature of the neoplasm by IHC (florescence). Could you please tell us which antibody that can specifically recognize human tissue (tumor) or mouse tissue (tumor) ? We hope that antibody does not cross-react on human or mouse. Also, the host of antibody is not form mouse.

ANSWER:

Thank you for contacting us. I have heard of people using anti-vimentin clone V9, to detect human tissue implanted in mice.

However, this is a mouse monoclonal and our version, ab8069 is unconjugated. We do not have a version conjugated to a fluorochrome, enzyme, or other detector. An anti-mouse IgG secondary may give some staining of endogenous IgG, as you are probably aware.

For detecting mouse tissue without detecting human, consider ab15630, with is also available biotinylated as ab15631 and FITC-conjugated as ab15638:

http://www.abcam.com/index.html?datasheet=15630

The ab15630 and ab15631 have been shown to work on frozen tissue, but will not work on paraffin-embedded tissue. Ab15638 has only been tested for flow cytometry so we canot guarantee it will work for IHC.

Antibody ab6022 has not been tested on mouse tissue. An alignment of the immunogenic peptide with the mouse protein shows that it is 69% identical; it is difficult to predict from this if the antibody will or will not cross-react with the mouse protein. The alignment can be found at this address for the next 24 hours:

http://www.ebi.ac.uk/cgi-bin/clustalw/result?tool=clustalw&jobid=clustalw-20070510-00025988&poll=yes

I hope this helps; please do not hesitate to contact us if you have any questions.