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Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)
Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)
(enlarge)


Product Name 

PAR6 antibody

 

See all PAR6 antibodies (5)...

Product type 

Primary antibodies

Description 

Goat polyclonal to PAR6

Immunogen 

Peptide with sequence GSRIRGDGSGFSL, from C Terminus of the protein sequence according to NP_058644
BLAST 'GSRIRGDGSGFSL' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:8,000.
IHC-P: Use at a concentration of 5 - 10 µg/ml.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human Brain and Pancreas extracts.

Cellular localization 

Cell Membrane

Research areas 

Stem Cells >> Cancer Stem Cells >> Intracellular
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation

Relevance 

PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.

 

PAR6 antibody (ab6022)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

50855    (Human)

GeneCard

    

GC16P067430    (Human)

Omim

    

607484    (Human)

SwissProt

    

Q9NPB6    (Human)

Unigene

    

112933    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

GenBank Accession Number – NP_058644.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAR6 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for PAR6 antibody images, references, products related to ab6022 and other tools.

 

PAR6 antibody images:

Product image  Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)

Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)

Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)

ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.

 
 

Search PubMed (MEDLINE) for references to PAR6

 

PAR6 antibody - more information

Customer reviews (feedback) regarding PAR6 antibody

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Price and availability of products related to PAR6 antibody

 

PAR6 antibody - related products:

 

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ab6566

  

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ab6739

  

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ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other PAR6 Antibodies

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PAR6 antibody - other tools:

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