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Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)
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Product Name
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PAR6 antibody
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See all PAR6 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to PAR6
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Immunogen
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Peptide with sequence GSRIRGDGSGFSL, from C Terminus of the protein sequence according to NP_058644
BLAST 'GSRIRGDGSGFSL' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: Antibody detection limit dilution 1:8,000.
IHC-P: Use at a concentration of 5 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human Brain and Pancreas extracts.
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Cellular localization
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Cell Membrane
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Research areas
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Stem Cells >> Cancer Stem Cells >> Intracellular
Cancer >> Cell cycle >> Cell division
Cell Biology >> Cell Cycle >> Cell Division >> Other cell division antibodies
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Differentiation
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Relevance
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PAR6 is a cell membrane protein involved in cell polarization and asymmetrical cell division processes as part of a multi protein complex. It is thought to be involved in the invasive phenotype that is associated with metastatic carcinomas, playing a role in the epithelial to mesenchymal transition (EMT). PAR6 is also thought to be involved in the formation of epithelial tight junctions. PAR6 contains a semi Cdc42/Rac interactive binding (CRIB) domain and a PDZ (PSD95/Discs-large/ZO1) domain, and several different PAR6 isoforms have been identified.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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GenBank Accession Number – NP_058644.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAR6 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAR6 antibody images, references, products related to ab6022 and other tools.
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PAR6 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - PAR6 antibody (ab6022)
Ab6022 (10ug/ml) staining human PAR6 in human pancreas by immunohistochemistry using paraffin embedded tissue. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
Ab6022 shows strong cytoplasmic staining in rare cells of intralobular ducts.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAR6 antibody (ab6022)
ab6022 at 5ug/ml staining PAR6 in human heart tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
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Search PubMed (MEDLINE) for references to PAR6
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PAR6 antibody - more information
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Customer reviews (feedback) regarding PAR6 antibody |
Customer FAQs regarding PAR6 antibody |
Protocols for PAR6 antibody |
Price and availability of products related to PAR6 antibody |
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PAR6 antibody - related products:
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PAR6 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAR6 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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