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Product name
Anti-PARC antibody
See all PARC products (5) ...
Description
Rabbit polyclonal to PARC
Tested applications
WB, IP, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: FFFGDEEEDEDEAYD conjugated to KLH, corresponding to C terminal amino acids 2503 - 2517 of Human PARC
FFFGDEEE DEDEAYD
Positive control
Most cell lines expressing PARC can be used as a positive control. HeLa and NHI 3T3 cell lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Purification notes
ab34847 is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> p53 pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 281 kDa (predicted molecular weight: 281 kDa).
IP: Use at an assay dependent dilution.
ELISA: 1/2000 - 1/10000.
Target
Function
Cytoplasmic anchor protein in p53-associated protein complex. Regulates the subcellular localization of p53 and subsequent function. Seems to be part of an atypical cullin-RING-based E3 ubiquitin-protein ligase complex. In vitro, complexes of CUL9/PARC with either CUL7 or TP53 contain E3 ubiquitin-protein ligase activity.
Tissue specificity
Ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Sequence similarities
Belongs to the cullin family.
Contains 1 DOC domain.
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers.
Domain
The IBR domain is required for interaction with UBCH7 and UBCH8.
Post-translational
modifications
In vitro, self-ubiquitination in the presence of E1, E2 and ubiquitin.
Neddylated.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ8IWT3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CUL-9 antibody
- Cul9 antibody
- CUL9_HUMAN antibody
see all
Database links
- Entrez Gene: 23113 Human
- Entrez Gene: 78309 Mouse
- Omim: 607489 Human
- SwissProt: Q8IWT3 Human
- SwissProt: Q80TT8 Mouse
- Unigene: 485434 Human
- Unigene: 311593 Mouse
- Unigene: 370382 Mouse
see all
References for Anti-PARC antibody (ab34847)
ab34847 has not yet been referenced specifically in any publications.
Publishing research using ab34847? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"