Overview

  • Product nameAnti-PARD3 antibodySee all PARD3 primary antibodies ...
  • Description
    Rabbit polyclonal to PARD3
  • Tested applicationsWB, ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from an internal region of Human PAR3.

  • Positive control
    • COLO205 or HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration. PubMed: 24312324

Target

  • FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PAR3 family.
    Contains 3 PDZ (DHR) domains.
  • DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
    The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
  • Post-translational
    modifications
    Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
    Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
  • Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • agouti signaling protein antibody
    • ASIP antibody
    • Atypical PKC isotype specific interacting protein antibody
    • Atypical PKC isotype-specific-interacting protein antibody
    • Baz antibody
    • bazooka antibody
    • Coagulation factor II receptor-like 2 antibody
    • CTCL tumor antigen se2-5 antibody
    • F2rl2 antibody
    • PAR-3 antibody
    • par-3 family cell polarity regulator alpha antibody
    • par-3 family cell polarity regulator antibody
    • PAR3 alpha antibody
    • par3 antibody
    • PAR3-alpha antibody
    • PARD-3 antibody
    • PARD3 antibody
    • PARD3_HUMAN antibody
    • PARD3A antibody
    • Partitioning defective 3 homolog antibody
    • Partitioning-defective 3 homolog antibody
    • Partitioning-defective protein 3, C. elegans, homolog of antibody
    • SE2-5L16 antibody
    • SE2-5LT1 antibody
    • SE2-5T2 antibody
    • Thrombin receptor-like 2 antibody
    see all

Anti-PARD3 antibody images

  • All lanes : Anti-PARD3 antibody (ab64840) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells
    Lane 2 : Extracts from COLO205 cells with blocking peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 151 kDa
    Observed band size : 151 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab64840 (1:500).

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

References for Anti-PARD3 antibody (ab64840)

This product has been referenced in:
  • Omri S  et al. PKC? Mediates Breakdown of Outer Blood-Retinal Barriers in Diabetic Retinopathy. PLoS One 8:e81600 (2013). IHC-P ; Rat . Read more (PubMed: 24312324) »
  • Tanner K  et al. Coherent angular motion in the establishment of multicellular architecture of glandular tissues. Proc Natl Acad Sci U S A 109:1973-8 (2012). ICC/IF ; Human . Read more (PubMed: 22308439) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (prostate cancer cells)
Loading amount 30 µg
Specification prostate cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 27 2012

Thanks for your enquiry! I will enquire with our supplying lab to determine if ab64840 may also cross react with zebrafish PARD3 and will forward you any information as soon as possible. As we discussed, please reply to this email to let me know if you...

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