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Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-PARD3 antibody
See all PARD3 products (3) ...
Description
Rabbit polyclonal to PARD3
Tested applications
WB, ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
Synthetic peptide from an internal region of Human PAR3.
Positive control
COLO205 or HepG2 cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ or Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Adapters >> Transmembrane
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Relevance
PARD3 is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PARD3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PARD3 interacts with PRCKI. PARD3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PARD3 interacts with F11R/JAM1. Antibodies against PARD3 are present in sera from patients with cutaneous T cell lymphomas.
Cellular localization
Cytoplasmic; membrane associated. Partially localized along the cell cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions.
Alternative names
- agouti signaling protein antibody
- ASIP antibody
- Atypical PKC isotype specific interacting protein antibody
see all
Database links
- Entrez Gene: 56288 Human
- Entrez Gene: 93742 Mouse
- Entrez Gene: 81918 Rat
- Omim: 606745 Human
- SwissProt: Q8TEW0 Human
- SwissProt: Q99NH2 Mouse
- SwissProt: Q9Z340 Rat
- Unigene: 131489 Human
Anti-PARD3 antibody images:
Western blot - PAR3 antibody (ab64840)
All lanes : Anti-PARD3 antibody (ab64840) at 1/500 dilution
Lane 1 : Extracts from COLO205 cells
Lane 2 : Extracts from COLO205 cells with blocking peptide at 5 µg
Lysates/proteins at 10 µg per lane.
Predicted band size : 151 kDa
Observed band size : 151 kDa
Immunocytochemistry/ Immunofluorescence - PAR3 antibody (ab64840)
Immunofluorescence analysis of HepG2 cells, using ab64840 (1:500).
The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.
References for Anti-PARD3 antibody (ab64840)
This product has been referenced in:
- Gómez-Flores Eet al. Asymmetrical cell division and differentiation are not dependent upon stratification in a corneal epithelial cell line. J Cell Physiol 226:700-9 (2011). ICC/IF; Human.Read more (PubMed: 20717959) »
See 1 publication for this product
Publishing research using ab64840? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"