You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameAnti-PARD3 antibodySee all PARD3 primary antibodies ...
  • Description
    Rabbit polyclonal to PARD3
  • Tested applicationsWB, ICC/IF, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide from an internal region of Human PAR3.

  • Positive controlCOLO205 or HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab64840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 151 kDa (predicted molecular weight: 151 kDa).
ICC/IF ICC/IF: 1/500 - 1/1000.
ELISA ELISA: 1/20000.

Target

  • FunctionAdapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesBelongs to the PAR3 family.
    Contains 3 PDZ (DHR) domains.
  • DomainContains a conserved N-terminal oligomerization domain (NTD) that is involved in oligomerization and is essential for proper subapical membrane localization.
    The second PDZ domain mediates interaction with membranes containing phosphoinositol lipids.
  • Post-translational
    modifications    Phosphorylated by PRKCZ. EGF-induced Tyr-1127 phosphorylation mediates dissociation from LIMK2.
    Phosphorylation by STK6/AURKA at Ser-962 is required for the normal establishment of neuronal polarity.
  • Cellular localizationEndomembrane system. Cell junction. Cell junction > tight junction. Cell membrane. Cytoplasm > cell cortex. Cytoplasm > cytoskeleton. Localized along the cell-cell contact region. Colocalizes with PARD6A and PRKCI at epithelial tight junctions. Colocalizes with the cortical actin that overlays the meiotic spindle during metaphase I and metaphase II (By similarity). Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.

    • Target information above from: UniProt accession Q8TEW0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      agouti signaling protein antibodyASIP antibodyAtypical PKC isotype specific interacting protein antibody
      Atypical PKC isotype-specific-interacting protein antibodyCoagulation factor II receptor-like 2 antibodyCTCL tumor antigen se2-5 antibodyF2rl2 antibodyPAR-3 antibodyPAR3 alpha antibodypar3 antibodyPAR3-alpha antibodyPARD-3 antibodyPARD3 antibodyPARD3_HUMAN antibodyPartitioning defective 3 homolog antibodyPartitioning-defective 3 homolog antibodyThrombin receptor-like 2 antibody
    see all

Anti-PARD3 antibody images

  • Western blot - PAR3 antibody (ab64840)
    Western blot - PAR3 antibody (ab64840)
    All lanes : Anti-PARD3 antibody (ab64840) at 1/500 dilution

    Lane 1 : Extracts from COLO205 cells
    Lane 2 : Extracts from COLO205 cells with blocking peptide at 5 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 151 kDa
    Observed band size : 151 kDa
  • Immunocytochemistry/ Immunofluorescence - PAR3 antibody (ab64840)
    Immunocytochemistry/ Immunofluorescence - PAR3 antibody (ab64840)
    Immunofluorescence analysis of HepG2 cells, using ab64840 (1:500).

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

References for Anti-PARD3 antibody (ab64840)

This product has been referenced in:
  • Tanner K  et al. Coherent angular motion in the establishment of multicellular architecture of glandular tissues. Proc Natl Acad Sci U S A 109:1973-8 (2012). ICC/IF ; Human . Read more (PubMed: 22308439) »
  • Gómez-Flores E  et al. Asymmetrical cell division and differentiation are not dependent upon stratification in a corneal epithelial cell line. J Cell Physiol 226:700-9 (2011). ICC/IF ; Human . Read more (PubMed: 20717959) »

See all 2 Publications for this product

Publishing research using ab64840 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab64840 Submit a Question for ab64840

Displaying 1 - 2 of 2 results for Abreviews and Q&A

10
Votes: Highest First

Western blot abreview for Anti-PARD3 antibody

 Average
Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (prostate cancer cells)
Loading amount 30 µg
Specification prostate cancer cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
abreview image
Username

Verified customer

Submitted Feb 27 2012

I would like to test ab64840 and/or ab64646 for reactivity to zebrafish samples using WB and IHC.

Thanks for your enquiry! I will enquire with our supplying lab to determine if ab64840 may also cross react with zebrafish PARD3 and will forward you any information as soon as possible. As we discussed, please reply to this email to let me know if you ar...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"