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ab16386
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab16060? Please let us know so that we can cite the reference in this datasheet
ab16060 has been referenced in 3 publications.
- Mukherjee A et al. Selective signaling by Akt2 promotes bone morphogenetic protein 2-mediated osteoblast differentiation. Mol Cell Biol 30:1018-27 (2010). WB; Human. PubMed: 19995912
- Farrar D et al. Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation. Mol Cell Biol 30:1199-216 (2010). WB; Human. PubMed: 20038529
- Fisher AE et al. Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase. Mol Cell Biol 27:5597-605 (2007). WB; Human. PubMed: 17548475
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PARG antibody (ab16060)
Lanes 1 - 5 : Anti-PARG antibody (ab16060)
Lanes 6 - 10 : Anti-PARG antibody (ab16060) at 0.1 µg
Lane 1 : Hela nuclear
Lane 2 : Hela WC
Lane 3 : A431
Lane 4 : Mouse Brain
Lane 5 : Mouse Liver
Lane 6 : Hela nuclear with
Lane 7 : Hela WC with
Lane 8 : A431 with
Lane 9 : Mouse Brain with
Lane 10 : Mouse Liver with
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) (1/10,000)
Predicted band size : 56,110, kDa
Observed band size : 56,110 kDa
ab16060 recognizes the ~59 kDa PARG protein. Also detects ~102-110 kDa higher molecular weight forms of PARG. The 59 kDa band is specifically blocked by the immunising peptide in all lysates.
Immunocytochemistry/ Immunofluorescence - PARG antibody (ab16060)
ICC/IF image of ab16060 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16060, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK293 and HepG2 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARG antibody (ab16060)
IHC image of PARG staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16060, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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