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Overview
Product name
Anti-PARG antibody
See all PARG products (3) ...
Description
Rabbit polyclonal to PARG
Tested applications
IHC-P, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PARG.
(Peptide available ab16386.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 5 µg/ml
WB: Use a concentration of 0.1 µg/mlDetects a band of approximately 56,110 kDa (predicted molecular weight: 56,110, kDa).
Target
Function
Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the poly(ADP-ribose) glycohydrolase family.
Cellular localization
Cytoplasm and Nucleus.
Target information above from: UniProt accessionQ86W56
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP riboseglycohydrolase antibody
- Parg antibody
- PARG_HUMAN antibody
see all
Database links
- Entrez Gene: 8505 Human
- Omim: 603501 Human
- SwissProt: Q86W56 Human
- Unigene: 10136 Human
- Unigene: 499594 Human
- Unigene: 535298 Human
Anti-PARG antibody images:
Western blot - PARG antibody (ab16060)
Lanes 1 - 5 : Anti-PARG antibody (ab16060)
Lanes 6 - 10 : Anti-PARG antibody (ab16060) at 0.1 µg
Lane 1 : Hela nuclear
Lane 2 : Hela WC
Lane 3 : A431
Lane 4 : Mouse Brain
Lane 5 : Mouse Liver
Lane 6 : Hela nuclear with PARG peptide (ab16386) at 1 µg/ml
Lane 7 : Hela WC with PARG peptide (ab16386) at 1 µg/ml
Lane 8 : A431 with PARG peptide (ab16386) at 1 µg/ml
Lane 9 : Mouse Brain with PARG peptide (ab16386) at 1 µg/ml
Lane 10 : Mouse Liver with PARG peptide (ab16386) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) (1/10,000)
Predicted band size : 56,110, kDa
Observed band size : 56,110 kDa
ab16060 recognizes the ~59 kDa PARG protein. Also detects ~102-110 kDa higher molecular weight forms of PARG. The 59 kDa band is specifically blocked by the immunising peptide in all lysates.
Immunocytochemistry/ Immunofluorescence - PARG antibody (ab16060)
ICC/IF image of ab16060 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16060, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK293 and HepG2 cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARG antibody (ab16060)
IHC image of PARG staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16060, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
References for Anti-PARG antibody (ab16060)
This product has been referenced in:
- Mukherjee Aet al. Selective signaling by Akt2 promotes bone morphogenetic protein 2-mediated osteoblast differentiation. Mol Cell Biol 30:1018-27 (2010). WB; Human.Read more (PubMed: 19995912) »
- Farrar Det al. Mutational analysis of the poly(ADP-ribosyl)ation sites of the transcription factor CTCF provides an insight into the mechanism of its regulation by poly(ADP-ribosyl)ation. Mol Cell Biol 30:1199-216 (2010). WB; Human.Read more (PubMed: 20038529) »
See all 3 publications for this product
Publishing research using ab16060? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"