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Western blot - PARG antibody (ab16060)
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Immunocytochemistry/ Immunofluorescence - PARG antibody (ab16060)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARG antibody (ab16060)
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Product Name
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PARG antibody
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See all PARG antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PARG
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PARG.
(Peptide available ab16386.)
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Reacts with
(species key)
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Hu
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Tested applications
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 0.1 µg/ml. Detects a band of approximately 56,110 kDa (predicted molecular weight: 56,110, kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear (isoform 1). Cytoplasmic (isoforms 2 and 3).
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
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Relevance
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PARG is the major enzyme responsible for the catabolism of poly(ADP-ribose), which is a reversible covalent-modifier of chromosomal proteins. The protein may be subject to proteolysis generating smaller, active products and is found in many tissues.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PARG antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PARG antibody images, references, products related to ab16060 and other tools.
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PARG antibody images:
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 Western blot - PARG antibody (ab16060)
Lanes 1 - 5 : PARG antibody (ab16060)
Lanes 6 - 10 : PARG antibody (ab16060) at 0.1 µg
Lane 1 : Hela nuclear
Lane 2 : Hela WC
Lane 3 : A431
Lane 4 : Mouse Brain
Lane 5 : Mouse Liver
Lane 6 : Hela nuclear with PARG peptide (ab16386) at 1 µg/ml
Lane 7 : Hela WC with PARG peptide (ab16386) at 1 µg/ml
Lane 8 : A431 with PARG peptide (ab16386) at 1 µg/ml
Lane 9 : Mouse Brain with PARG peptide (ab16386) at 1 µg/ml
Lane 10 : Mouse Liver with PARG peptide (ab16386) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) (1/10,000)
Predicted band size : 56,110, kDa
Observed band size : 56,110 kDa
ab16060 recognizes the ~59 kDa PARG protein. Also detects ~102-110 kDa higher molecular weight forms of PARG. The 59 kDa band is specifically blocked by the immunising peptide in all lysates.
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Immunocytochemistry/ Immunofluorescence - PARG antibody (ab16060)
ICC/IF image of ab16060 stained human MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab16060, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK293 and HepG2 cells.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARG antibody (ab16060)
IHC image of PARG staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16060, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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References for PARG antibody (ab16060)
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This product has been used in:
Fisher AE et al. Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase. Mol Cell Biol 27:5597-605 (2007). WB; Human. PubMed: 17548475
If you publish research using ab16060 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to PARG
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PARG antibody - more information
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Customer reviews (feedback) regarding PARG antibody |
Customer FAQs regarding PARG antibody |
Protocols for PARG antibody |
Price and availability of products related to PARG antibody |
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PARG antibody - related products:
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PARG antibody - other tools:
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Contact Abcam with a Technical Enquiry about PARG antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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