Anti-PARG antibody (ab43412)

Product name

Anti-PARG antibody
See all PARG products (3) ...

Description

Mouse polyclonal to PARG

Tested applications

WBmore details

Cross reactivity

Reacts with

Fruit fly (Drosophila melanogaster)

Immunogen

Recombinant fusion protein: SYSRLIKEKS SKEPRENKAS KKKLYDFIKE ELKKVRDVPG EGASAEAGSS RVAGLGEGKS ETSAKSSPEL NKQPARPQIT ITQQSTDLLP AQLSQDNSNS , corresponding to amino acids 566-665 of Fruit fly (Drosophila melanogaster) PARG

SYSRLIKEKS SKEPRENKAS KKKLYDFIKE ELKKVRDVPG EGASAEAGSS RVAGLGEGKS ETSAKSSPEL NKQPARPQIT ITQQSTDLLP AQLSQDNSNS

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Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol

Purity

Whole antiserum

Primary antibody notes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation