Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Parkin / PARK
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ab4150 has been referenced in 7 publications.
Publishing research using ab4150? Please let us know so that we can cite the reference in this datasheet
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Predicted band size : 20 kDa
ab4150 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Predicted band size : 20 kDa
Western blot using ab4150 at 1/500.
Exposure time: 30s.
Left hand lane: HeLa whole cell lysate (human)
Right hand lane: 3T3 whole cell lysate (mouse)
Anti-PARK7/DJ1 antibody (ab4150) at 1/6000 dilution
Secondary
HRP conjugated Donkey anti mouse at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 20 kDa
Observed band size : 24 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
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