Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

- PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

Western blot using clone malphaDJ-1/E2.19 and a beta actin antibody as a loading control.

The bottom band is PARK7/DJ1, the top band is beta actin.

Lane 1: 293 cell lysate

Lane 2: MCF-7 cell lysate

Lanes 3-7: various different prostate cell lines

Lane 8: recombinant PARK7/DJ1 (that was used as immunogen for this antibody)

Western blot - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

Western blot using ab11251 at 1/500 on HeLa whole cell lysate (20µg/lane).

Immunocytochemistry/ Immunofluorescence - PARK7/DJ1 antibody [malphaDJ-1/E2.19] (ab11251)

ICC/IF image of ab11251 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab11251, 1:500 dilution) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Flow Cytometry-Anti-PARK7/DJ1 antibody [malphaDJ-1/E2.19](ab11251)

Overlay histogram showing HepG2 cells stained with ab11251 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11251, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.