Anti-PARL antibody (ab42638)

Overview

• Product nameAnti-PARL antibodySee all PARL primary antibodies ...
• Description
  Rabbit polyclonal to PARL
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human PARL.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab45229.)

• Positive controlab42638 gave a positive result in Jurkat Whole cell Lysate and Hela Whole Cell Lysate (data not shown).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Proteases

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Alzheimer's disease
  • Other

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Other proteases

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionRequired for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity). Promotes changes in mitochondria morphology regulated by phosphorylation of P-beta domain.
• Sequence similaritiesBelongs to the peptidase S54 family.
• Post-translational
  modificationsP-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner. The cleavage is inhibited when residues Ser-65, Thr-69 and Ser-70 are all phosphorylated.
• Cellular localizationMitochondrion inner membrane and Nucleus. Translocated into the nucleus by an unknown mechanism.

Target information above from: UniProt accession Q9H300 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 514191 Cow
  • Entrez Gene: 55486 Human
  • Entrez Gene: 55486 Human
  • Entrez Gene: 55486 Human
  • Entrez Gene: 381038 Mouse
  • Entrez Gene: 287979 Rat
  • Omim: 607858 Human
  • SwissProt: Q2KHV4 Cow

  • SwissProt: Q9H300 Human
  • SwissProt: Q9H300 Human
  • SwissProt: Q9H300 Human
  • SwissProt: Q5XJY4 Mouse
  • SwissProt: Q3B8P0 Rat
  • Unigene: 478469 Human
  • Unigene: 371737 Mouse
  • Unigene: 7053 Rat

  see all

• Alternative names
  Mitochondrial intramembrane cleaving protease PARL antibodyMitochondrial intramembrane-cleaving protease PARL antibodyP-beta antibody

  parl antibodyPARL_HUMAN antibodyPbeta antibodypresenilin associated rhomboid-like antibodyPresenilins associated rhomboid-like protein antibodyPRO2207 antibodyPSARL antibodyPSARL1 antibodyPSENIP2 antibodyRHBDS1 antibodyRhomboid 7 homolog 1 antibody

  see all

Anti-PARL antibody images

• 

  Western blot - PARL antibody (ab42638)
  Anti-PARL antibody (ab42638) at 1 µg/ml + Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  Additional bands at : 110 kDa,36 kDa (possible cleavage fragment),40 kDa,60 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunocytochemistry/ Immunofluorescence - PARL antibody (ab42638)
  ICC/IF image of ab42638 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab42638, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

• 

  Western blot - PARL antibody (ab42638)
  All lanes : Anti-PARL antibody (ab42638) at 1/1000 dilution
  
  Lane 1 : Lysate prepared from mitochondria isolated from HEK293T cells transfected with non-targeting siRNA.
  Lane 2 : Lysate prepared from mitochondria isolated from HEK293T cells transfected with PARL-specific siRNA.
  
  Lysates/proteins at 24 µg per lane.
  
  Secondary
  Goat anti-rabbit IgG conjugated to HRP at 1/5000 dilution
  developed using the ECL technique
  
  Predicted band size : 42 kDa
  Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 32 kDa,33 kDa,38 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 10 seconds

  Image courtesy of Andrew Greene by Abreview.

  See Abreview