Anti-PARN antibody (ab125185)
Key features and details
- Rabbit polyclonal to PARN
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PARN antibody
See all PARN primary antibodies -
Description
Rabbit polyclonal to PARN -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 589-639 of Human PARN (NP_002573.1).
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Positive control
- WB: HeLa, Jurkat and 293T whole cell lysates. IP: HeLa cell lysate. IHC-P: Human breast carcinoma and mouse renal carcinoma tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 73 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 73 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the CAF1 family.
Contains 1 R3H domain. -
Cellular localization
Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar. - Information by UniProt
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Database links
- Entrez Gene: 453936 Chimpanzee
- Entrez Gene: 5073 Human
- Entrez Gene: 74108 Mouse
- Entrez Gene: 100172259 Orangutan
- Omim: 604212 Human
- SwissProt: O95453 Human
- SwissProt: Q8VDG3 Mouse
- SwissProt: Q5RC51 Orangutan
see all -
Alternative names
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
see all
Images
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All lanes : Anti-PARN antibody (ab125185) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 73 kDa
Exposure time: 3 minutes -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling PARN with ab125185 at a 1/1000 dilution.
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ab125185, at 6 µg/mg lysate, detecting PARN in HeLa cell lysate (1 mg of immunoprecipitate, 20% loaded in the lane) by Immunoprecipitation.
For the subsequent Western blot, ab125185 was used at 1 µg/ml.
The blot was developed using the ECL technique, with an exposure time of 3 seconds. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse renal carcinoma tissue labeling PARN with ab125185 at a 1/1000 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab125185 has been referenced in 1 publication.
- Duan TL et al. Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN). Cells 9:N/A (2020). PubMed: 31936572