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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-PARN antibody
See all PARN products (3) ...
Description
Rabbit polyclonal to PARN
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.
(Peptide available as ab28513.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27778 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/100 (please see abreview).
IHC-P: 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localization
Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
Target information above from: UniProt accessionO95453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
see all
Database links
- Entrez Gene: 5073 Human
- Omim: 604212 Human
- SwissProt: O95453 Human
- Unigene: 253197 Human
Anti-PARN antibody images:
Western blot - PARN antibody (ab27778)
All lanes : Anti-PARN antibody (ab27778) at 1 µg/ml
Lane 1 : HeLa Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 73 kDa
Immunocytochemistry/ Immunofluorescence - PARN antibody (ab27778)
ab27778, at 1/100 dilution, staining human PARN in HeLa cells.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARN antibody (ab27778)
Image courtesy of Human Protein Atlas
ab27778 staining PARN in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab27778 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab27778 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
References for Anti-PARN antibody (ab27778)
ab27778 has not yet been referenced specifically in any publications.
Publishing research using ab27778? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"