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Western blot - PARN antibody (ab27778)
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Immunocytochemistry/ Immunofluorescence - PARN antibody (ab27778)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARN antibody (ab27778)
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Product Name
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PARN antibody
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See all PARN antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PARN
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.
(Peptide available as ab28513.)
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Reacts with
(species key)
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Hu
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Tested applications
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/100 (please see abreview).
IHC-P: 1/25. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nucleus and cytoplasm. Some nuclear fraction is nucleolar.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Relevance
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A 3' exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. It interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. It is involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. It is also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3' untranslated regions, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (referenced from swissprot).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.40 - 0.80 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PARN antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PARN antibody images, references, products related to ab27778 and other tools.
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PARN antibody images:
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 Immunocytochemistry/ Immunofluorescence - PARN antibody (ab27778)
ab27778, at 1/100 dilution, staining human PARN in HeLa cells. This image is courtesy of an Abreview submitted by Dr Kirk McManus
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PARN antibody (ab27778)
Image courtesy of Human Protein Atlas
ab27778 staining PARN in human small intestine. Paraffin embedded human small intestine tissue was incubated with ab27778 (1/25 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab27778 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
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Search PubMed (MEDLINE) for references to PARN
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PARN antibody - more information
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Customer reviews (feedback) regarding PARN antibody |
Customer FAQs regarding PARN antibody |
Protocols for PARN antibody |
Price and availability of products related to PARN antibody |
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PARN antibody - related products:
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PARN antibody - other tools:
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Contact Abcam with a Technical Enquiry about PARN antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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