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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-PARN antibody
See all PARN products (3) ...
Description
Rabbit polyclonal to PARN
Tested applications
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Cow, Dog
Immunogen
Synthetic peptide: PSVTALTSGF DTPEERYQKL KKHSMDFLLF QFGLCAFKYD HTDSKHVTKS , corresponding to amino acids 38-87 of Human PARN
PSVTALTSGF DTPEERYQKL KKHSMDFLLF QFGLCAFKYD HTDSKHVTKS
Positive control
SP2/0 lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
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Western blot - PARN antibody (ab66623)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 59 , 80 kDa (predicted molecular weight: 69 kDa).
Target
Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localization
Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
Target information above from: UniProt accessionO95453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
see all
Database links
- Entrez Gene: 524155 Cow
- Entrez Gene: 479839 Dog
- Entrez Gene: 74108 Mouse
- Entrez Gene: 360464 Rat
- SwissProt: P69341 Cow
- SwissProt: Q8VDG3 Mouse
- Unigene: 15863 Cow
- Unigene: 182350 Mouse
see all
Anti-PARN antibody images:
Western blot - PARN antibody (ab66623)
Anti-PARN antibody (ab66623) at 1.25 µg/ml + SP2/0 lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 69 kDa
Observed band size : 59,80 kDa (why is the actual band size different from the predicted?)
References for Anti-PARN antibody (ab66623)
ab66623 has not yet been referenced specifically in any publications.
Publishing research using ab66623? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"