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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Overview
Product name
Anti-PARN antibody
See all PARN products (4) ...
Description
Mouse polyclonal to PARN
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Full length human PARN protein (NP_002573.1).
Positive control
WB: HeLa nuclear extract, Raw 264.7 and NIH/3T3 PC12 cell lysates; PARN transfected 293T cell lysate ICC/IF: HeLa cell
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1 x PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab89831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 71 kDa.
ICC/IF: Use a concentration of 10 µg/ml
Target
Function
3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the CAF1 family.
Contains 1 R3H domain.
Cellular localization
Nucleus. Cytoplasm. Nucleus > nucleolus. Some nuclear fraction is nucleolar.
Target information above from: UniProt accessionO95453
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DAN antibody
- Deadenylating nuclease antibody
- Deadenylation nuclease antibody
see all
Database links
- Entrez Gene: 5073 Human
- Entrez Gene: 74108 Mouse
- Entrez Gene: 360464 Rat
- Omim: 604212 Human
- SwissProt: O95453 Human
- SwissProt: Q8VDG3 Mouse
- Unigene: 253197 Human
- Unigene: 182350 Mouse
see all
Anti-PARN antibody images:
Western blot - PARN antibody (ab89831)
Anti-PARN antibody (ab89831) at 1 µg/ml + PC12 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Western blot - PARN antibody (ab89831)
Anti-PARN antibody (ab89831) at 1 µg/ml + HeLa nuclear extract at 50 µg
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Western blot - PARN antibody (ab89831)
Anti-PARN antibody (ab89831) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~22 kDa,~25 kDa,~28 kDa,~30 kDa,~35 kDa,~45 kDa. We are unsure as to the identity of these extra bands.
Western blot - PARN antibody (ab89831)
Anti-PARN antibody (ab89831) at 1 µg/ml + NIH/3T3 cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~120 kDa. We are unsure as to the identity of these extra bands.
Western blot - PARN antibody (ab89831)
All lanes : Anti-PARN antibody (ab89831) at 1 µg/ml
Lane 1 : PARN transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
developed using the ECL technique
Predicted band size : 71 kDa
Observed band size : ~85 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - PARN antibody (ab89831)
ab89831, at 10 µg/ml, staining PARN in paraformaldehyde fixed, permeabilised HeLa cell by immunofluorescence.
References for Anti-PARN antibody (ab89831)
ab89831 has not yet been referenced specifically in any publications.
Publishing research using ab89831? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"