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ab32138 has been referenced in 2 publications.
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All lanes : Anti-PARP antibody [E102] (ab32138) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + Camptothecin cell lysate
Predicted band size : 113 kDa
Observed band size : 25,120 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of PARP expression in paraffin embedded human brain tissue section, using 1/25 ab32138.
Overlay histogram showing Jurkat cells stained with ab32138 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32138, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
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