![Western blot - PARP antibody [E102] (ab32138) image](#WB PARP.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - PARP antibody [E102] (ab32138) image](#IHC PARP.jpg)
 image](#PARP-Primary-antibodies-ab32138-1.jpg)
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Overview
Product name
Anti-PARP antibody [E102]
See all PARP products (13) ...
Description
Rabbit monoclonal [E102] to PARP
Specificity
ab32138 recognises both pro-form and p25 cleaved form of PARP.
Tested applications
WB, IHC-P, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to N terminal residues of human PARP
Positive control
Jurkat cell lysate, human brain tissue
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E102
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000Predicted molecular weight: 113 kDa.
IHC-P: 1/25
ICC
ICC: 1/100ICC: 1/100
Flow Cyt: 1/50
Application notes
Is unsuitable for or IP.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase NAD+ antibody
- ADP ribosyltransferase NAD+ antibody
see all
Database links
- Entrez Gene: 142 Human
- Entrez Gene: 11545 Mouse
- Entrez Gene: 25591 Rat
- Omim: 173870 Human
- SwissProt: P09874 Human
- SwissProt: P11103 Mouse
- SwissProt: P27008 Rat
- Unigene: 177766 Human
see all
Anti-PARP antibody [E102] images:
Western blot - PARP antibody [E102] (ab32138)
![Western blot - PARP antibody [E102] (ab32138)](/ps/datasheet/Images/32/ab32138/WB PARP.jpg)
All lanes : Anti-PARP antibody [E102] (ab32138) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + Camptothecin cell lysate
Predicted band size : 113 kDa
Observed band size : 25,120 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Paraffin-embedded sections) - PARP antibody [E102] (ab32138)
![Immunohistochemistry (Paraffin-embedded sections) - PARP antibody [E102] (ab32138)](/ps/datasheet/Images/32/ab32138/IHC PARP.jpg)
Immunohistochemical analysis of PARP expression in paraffin embedded human brain tissue section, using 1/25 ab32138.
Flow Cytometry-PARP antibody [E102](ab32138)
](/ps/datasheet/images/32/ab32138/PARP-Primary-antibodies-ab32138-1.jpg)
Overlay histogram showing Jurkat cells stained with ab32138 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32138, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (0.5µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-PARP antibody [E102] (ab32138)
This product has been referenced in:
- Walldorf Jet al. Propranolol impairs liver regeneration after partial hepatectomy in C57Bl/6-mice by transient attenuation of hepatic lipid accumulation and increased apoptosis. Scand J Gastroenterol 45:468-76 (2010). WB; Mouse.Read more (PubMed: 20082593) »
- Demerjian Met al. Acute modulations in permeability barrier function regulate epidermal cornification: role of caspase-14 and the protease-activated receptor type 2. Am J Pathol 172:86-97 (2008). WB; Mouse.Read more (PubMed: 18156206) »
See all 2 publications for this product
Publishing research using ab32138? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"