Immunocytochemistry/ Immunofluorescence abreview for Anti-PARP antibody [E102]

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Abreviews
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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Other product details

Dilution 1/200
Incubation time 1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution 1/200

Additional data

Additional Notes Asynchronous cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong, nuclear-enriched staining pattern under these conditions. MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.
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Dr. Kirk Mcmanus

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Submitted May 31 2012