Western blot abreview for Anti-PARP antibody [E102]

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Abreviews
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Application Western blot
Sample Mouse Tissue lysate - whole (Kidney)
Loading amount 56 µg
Specification Kidney
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycin)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution 1/1000
Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST

Secondary antibody

Name Non-Abcam antibody was used: 7074S anti-rabbit IgG - Cell signaling
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution 1/1000

Detection

Detection method EZ-ECL
Exposure 5 second(s)
Bands Specific: between 100-140 kDa
Positive control blotted the same membrane with actin afterwards.

Additional data

Additional Notes Lysis buffer: Ripa + Protease inhibitors cocktail (Sigma), homogenised
transfer membrane used: NC BLOTTING MEMBRANE 0.22µ
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Submitted May 16 2013