Western blot abreview for Anti-PARP1 antibody [E102]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin)
Loading amount
56 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: 7074S anti-rabbit IgG - Cell signaling
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
EZ-ECL
Exposure
5 second(s)
Bands
Specific: between 100-140 kDa
Positive control
blotted the same membrane with actin afterwards.

Additional data

Additional Notes
Lysis buffer: Ripa + Protease inhibitors cocktail (Sigma), homogenised transfer membrane used: NC BLOTTING MEMBRANE 0.22´
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Submitted May 16 2013

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