Anti-PARP antibody [E78] (ab32071)

Dear Sir/Madam

The customer would like to PARP antibody that result same with the publication (page 1387, attach file) which have PARP protein and cleave form. So, please kindly recommend abcam antibody.

ANSWER:

Thank you for contacting us.

We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react.

If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples.

We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal).

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

We are having difficulty with the antibody ab32071-100 PARP (E78), lot No.191268. My colleague has tried the recommended 1:1000 dilution and 1:500 in a Western Blot with Hela cell extract but saw no signal. An actin antibody confirmed the extract was good. Have you had any other difficulties with this batch? Normally we are very happy with Abcam antibodies.

ANSWER:

I'm sorry to hear you are experiencing problems with ab32071.

You are the only person so far who has had problems with this product. There may be a number of reasons why the antibody is not working at the moment and I would like to offer to look at your protocol in details to see if the problem could be solved with a few modifications. I enclose below a link to a form to help you put your protocol information easily together,

http://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=32071&mode=questionaire

I would also recommend to run a positive control of Jurkat cell lysate to make sure that the problem is not due to low levels of the proteins in HeLa cells.

Please be assured that if the problem is due to the quality of the antibody we would be happy to offer you a refund or replacement vial if the antibody was purchased in the last 90 days.