![Western blot - PARP antibody [E78] (ab32071) image](#ab32071_1.jpg)
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Overview
Product name
Anti-PARP antibody [E78]
See all PARP products (13) ...
Description
Rabbit monoclonal [E78] to PARP
Specificity
ab32071 should recognise both pro-form and p85 cleaved-form of PARP-1.
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues near to C-terminal residues of human PARP-1
Positive control
Jurkat cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
E78
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
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Western blot - PARP antibody [E78] (ab32071)
![Western blot - PARP antibody [E78] (ab32071) image](/ps/datasheet/Images/32/ab32071/ab32071_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab32071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Detects a band of approximately 85 and 116 kDa (predicted molecular weight: 113 kDa).
Is unsuitable for FACS, ICC, IHC-P or IP.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase NAD+ antibody
- ADP ribosyltransferase NAD+ antibody
see all
Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: P09874 Human
- Unigene: 177766 Human
Anti-PARP antibody [E78] images:
Western blot - PARP antibody [E78] (ab32071)
All lanes : Anti-PARP antibody [E78] (ab32071) at 1/1000 dilution
Lane 1 : Jurkat cells
Lane 2 : Jurkat + Staurosporine
Predicted band size : 113 kDa
References for Anti-PARP antibody [E78] (ab32071)
ab32071 has not yet been referenced specifically in any publications.
Publishing research using ab32071? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"