![Western blot - PARP antibody [Y17] (ab32378) image](#ab32378.jpg)
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Product name
Anti-PARP antibody [Y17]
See all PARP products (12) ...
Description
Rabbit monoclonal [Y17] to PARP
Specificity
This antibody is specific to PARP. It should recognize the intact form (116kDa) and the p25 cleaved form of PARP.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Chinese Hamster
Does not react with
Mouse, Rat
Immunogen
A synthetic peptide corresponding to residues before the cleavage site of human PARP.
Positive control
WB: Jurkat and Camptothecin cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
Y17
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> DNA Damage Recognition
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Base Excision Repair
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> ADP-ribosylation
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Western blot - PARP antibody [Y17] (ab32378)
![Western blot - PARP antibody [Y17] (ab32378) image](/ps/datasheet/Images/32/ab32378/ab32378.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab32378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: 1/80.
WB: 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 113 kDa).
Is unsuitable for FACS, ICC or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150.
Sequence similarities
Contains 1 BRCT domain.
Contains 1 PARP alpha-helical domain.
Contains 1 PARP catalytic domain.
Contains 2 PARP-type zinc fingers.
Post-translational
modifications
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR.
Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.
S-nitrosylated, leading to inhibit transcription regulation activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP09874
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase) antibody
- ADP ribosyltransferase NAD+ antibody
- ADPRT 1 antibody
see all
Database links
- Entrez Gene: 142 Human
- Omim: 173870 Human
- SwissProt: Q9R152 Chinese Hamster
- SwissProt: P09874 Human
- Unigene: 177766 Human
Anti-PARP antibody [Y17] images:
Western blot - PARP antibody [Y17] (ab32378)
All lanes : Anti-PARP antibody [Y17] (ab32378) at 1/1000 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : Jurkat cell lysate and Camptothecin cell lysate.
Predicted band size : 113 kDa
Observed band size : 115 kDa (why is the actual band size different from the predicted?)
References for Anti-PARP antibody [Y17] (ab32378)
ab32378 has not yet been referenced specifically in any publications.
Publishing research using ab32378? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"