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ab45360 |
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ab93465 |
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Dear Sir/Madam
The customer would like to PARP antibody that result same with the publication (page 1387, attach file) which have PARP protein and cleave form. So, please kindly recommend abcam antibody. |
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ANSWER: |
Thank you for contacting us.
We have several antibodies which can be used to detect PARP in Western blotting. From the study I believe you wish to detect both the cleaved (C-terminal fragment) and the full length PARP form in mouse samples? This can be done using ab75757 (Chicken polyclonal) or ab37722 (Rabbit polyclonal). Although ab37722 has not been tested with mouse samples due but to the similarity in immunogen sequence (100%) it is predicted to react.
If however you are interested in human samples this can be achieved with ab32071 (Rabbit monoclonal [E78]) which was raised against a synthetic peptide of the C-teminal residues of human PARP. Please note this antibody does not react with mouse samples.
We also have several antibodies that specifically recognise the cleavage product of PARP such as ab4830 (reacts with human C-terminal PARP) and ab32064 (reacts with mouse and human PARP, N-terminal).
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-PARP antibody (ab37722) at 3 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 113 kDa
Observed band size : 113 kDa
Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
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