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Anti-PATJ antibody (ab84932) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 196 kDa
Observed band size : >168 kDa (why is the actual band size different from the predicted?)
Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
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