Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
Overview
Product name
Anti-PATJ antibody
See all PATJ products (2) ...
Description
Rabbit polyclonal to PATJ
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within N terminal amino acids 540-589 (EVMVATLDTQ IADDAELQKY SKLLPIHTLR LGVEVDSFDG HHYISSIVSG) of Human PATJ (NP_795352).
EVMVATLDTQ IADDAELQKY SKLLPIHTLR LGVEVDSFDG HHYISSIVSG
Positive control
293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cell Adhesion >> Tight Junctions
Neuroscience >> Neurotransmission >> Intracellular Signaling >> Adapters
-
Western blot - PATJ antibody (ab84932)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84932 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 196 kDa.
Target
Relevance
PATJ contains multiple PDZ domains. PDZ domains mediate protein-protein interactions, and proteins with multiple PDZ domains often organize multimeric complexes at the plasma membrane. PATJ localizes to tight junctions and to the apical membrane of epithelial cells. A similar protein in Drosophila is a scaffolding protein which tethers several members of a multimeric signaling complex in photoreceptors.
Cellular localization
Membrane. Cell junction; tight junction. Apical cell membrane. Note: Localized in the paranodal region of myelinating Schwann cells. Membrane-associated. Localizes to tight junctions in epithelial cells. Also found at the apical plasma membrane.
Alternative names
- Channel interacting PDZ domain protein antibody
- Cipp antibody
- FLJ26982 antibody
see all
Database links
- Entrez Gene: 10207 Human
- Entrez Gene: 12695 Mouse
- Entrez Gene: 313382 Rat
- Omim: 603199 Human
- SwissProt: Q8NI35 Human
- SwissProt: Q63ZW7 Mouse
Anti-PATJ antibody images:
Western blot - PATJ antibody (ab84932)
Anti-PATJ antibody (ab84932) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 196 kDa
Observed band size : >168 kDa (why is the actual band size different from the predicted?)
Additional bands at : 120 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
References for Anti-PATJ antibody (ab84932)
ab84932 has not yet been referenced specifically in any publications.
Publishing research using ab84932? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"