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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab12000? Please let us know so that we can cite the reference in this datasheet
ab12000 has been referenced in 3 publications.
- Alkhatib A et al. FoxO1 induces Ikaros splicing to promote immunoglobulin gene recombination. J Exp Med 209:395-406 (2012). PubMed: 22291095
- Nebral K et al. Incidence and diversity of PAX5 fusion genes in childhood acute lymphoblastic leukemia. Leukemia : (2008). WB; Human. PubMed: 19020546
- Bousquet M et al. A novel PAX5-ELN fusion protein identified in B-cell acute lymphoblastic leukemia acts as a dominant negative on wild-type PAX5. Blood 109:3417-23 (2007). PubMed: 17179230
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PAX5 antibody (ab12000)
Predicted band size : 42 kDa
Detection of PAX5 by Western blot analysis. PAX5 is detected in nuclear extracts derived from the Raji cell line using PAX5 rabbit polyclonal antibody at a 1/1000 dilution. This signal is diminished (lane 2) when the reaction mixture is incubated with PAX5 immunizing peptide.
Western blot - PAX5 antibody (ab12000)
All lanes : Anti-PAX5 antibody (ab12000) at 1/500 dilution
Lane 1 : SUPB15: human CD19+ pre-B ALL cell line
Lane 2 : SUPB15: human CD19+ pre-B ALL cell line
Lane 3 : 38B9 (murine pre-B cell line)
Lane 4 : 38B9 (murine pre-B cell line)
Lane 5 : 38B9 (murine pre-B cell line)
Lane 6 : Murine fetal liver CD19+ preB cells population no. 1
Lane 7 : Murine fetal liver CD19+ preB cells population no. 2
Lane 8 : Murine fetal liver CD19+ preB cells population no. 3
Lane 9 : Murine fetal liver CD19+ preB cells population no. 4
Lane 10 : 38B9 (murine pre-B cell line)
Lane 11 : HL60 (human AML-cell line: negative control)
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
As murine samples to be tested, we employed 2 different mouse precursor B cell populations: 38B9 (murine pre-B cell line) and a primary culture of CD19+ pre-B cells purified from mouse fetal liver. All lysates are whole cell lysates in RIPA buffer. A 10% gel PVDF membrane was used under denaturing conditions. Blocking was performed using 5% milk for 1 hour at 22°C.
This image was kindly supplied by Mrs Grazia Fazio by Abreview
PAX5 antibody for WB in Human (12000)
PAX5 antibody for ICC/IF in Human (12000)
PAX5 antibody for WB in Mouse (12000)
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