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Overview
Product name
Anti-PAX5 antibody
See all PAX5 products (15) ...
Description
Rabbit polyclonal to PAX5
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide: MDLEKNYPTPRTSRT, corresponding to amino acids 1-15 of Human PAX5.
MDLEKNYPTP RTSRT
Positive control
Raji nuclear extract.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide and 0.01% Thiomerosal
Constituents: PBS containing 0.2% Gelatin
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
Neuroscience >> Neurology process >> Neurogenesis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 50 kDa (predicted molecular weight: 42 kDa).
ICC/IF: Use at an assay dependent dilution. (See Abreview.)
Target
Function
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in disease
Note=A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Sequence similarities
Contains 1 paired domain.
Developmental stage
Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
Post-translational
modifications
O-glycosylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02548
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
see all
Database links
- Entrez Gene: 5079 Human
- Entrez Gene: 18507 Mouse
- Omim: 167414 Human
- SwissProt: Q02548 Human
- SwissProt: Q02650 Mouse
- Unigene: 654464 Human
- Unigene: 439659 Mouse
Anti-PAX5 antibody images:
Western blot - PAX5 antibody (ab12000)
Predicted band size : 42 kDa
Detection of PAX5 by Western blot analysis. PAX5 is detected in nuclear extracts derived from the Raji cell line using PAX5 rabbit polyclonal antibody at a 1/1000 dilution. This signal is diminished (lane 2) when the reaction mixture is incubated with PAX5 immunizing peptide.
Western blot - PAX5 antibody (ab12000)
All lanes : Anti-PAX5 antibody (ab12000) at 1/500 dilution
Lane 1 : SUPB15: human CD19+ pre-B ALL cell line
Lane 2 : SUPB15: human CD19+ pre-B ALL cell line
Lane 3 : 38B9 (murine pre-B cell line)
Lane 4 : 38B9 (murine pre-B cell line)
Lane 5 : 38B9 (murine pre-B cell line)
Lane 6 : Murine fetal liver CD19+ preB cells population no. 1
Lane 7 : Murine fetal liver CD19+ preB cells population no. 2
Lane 8 : Murine fetal liver CD19+ preB cells population no. 3
Lane 9 : Murine fetal liver CD19+ preB cells population no. 4
Lane 10 : 38B9 (murine pre-B cell line)
Lane 11 : HL60 (human AML-cell line: negative control)
Lysates/proteins at 10 µg per lane.
Secondary
HRP conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 42 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
As murine samples to be tested, we employed 2 different mouse precursor B cell populations: 38B9 (murine pre-B cell line) and a primary culture of CD19+ pre-B cells purified from mouse fetal liver. All lysates are whole cell lysates in RIPA buffer. A 10% gel PVDF membrane was used under denaturing conditions. Blocking was performed using 5% milk for 1 hour at 22°C.
This image was kindly supplied by Mrs Grazia Fazio by Abreview
References for Anti-PAX5 antibody (ab12000)
This product has been referenced in:
- Nebral Ket al. Incidence and diversity of PAX5 fusion genes in childhood acute lymphoblastic leukemia. Leukemia : (2008). WB; Human.Read more (PubMed: 19020546) »
- Bousquet Met al. A novel PAX5-ELN fusion protein identified in B-cell acute lymphoblastic leukemia acts as a dominant negative on wild-type PAX5. Blood 109:3417-23 (2007).Read more (PubMed: 17179230) »
See all 2 publications for this product
Publishing research using ab12000? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"