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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAX5 antibody (ab15164)
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Product Name
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PAX5 antibody
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See all PAX5 antibodies (10)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PAX5
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Immunogen
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Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Immunohistology (Formalin/paraffin): 1/50 for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Tonsil
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
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Relevance
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The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
PAX5 expression is not only continuously required for B cell lineage commitment during early B cell development but also for B lineage maintenance. PAX5 is found in most cases of mature and precursor B cell non-Hodgkin lymphomas/leukemias. Pax-5 is not detected in Multiple myeloma and solitary plasmacytoma cell lymphomas do express Pax5 except for those with terminal B cell differentiation. T cell neoplasms do not stain with anti-Pax5; however there is a strong
association with CD20 expression.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PAX5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PAX5 antibody images, references, products related to ab15164 and other tools.
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PAX5 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAX5 antibody (ab15164)
ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).
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Search PubMed (MEDLINE) for references to PAX5
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PAX5 antibody - more information
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Customer reviews (feedback) regarding PAX5 antibody |
Customer FAQs regarding PAX5 antibody |
Protocols for PAX5 antibody |
Price and availability of products related to PAX5 antibody |
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PAX5 antibody - related products:
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PAX5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PAX5 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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