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Overview
Product name
Anti-PAX5 antibody
See all PAX5 products (15) ...
Description
Rabbit polyclonal to PAX5
Tested applications
WB, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide from C-terminus of human Pax5 protein (the amino acid sequence is considered to be commercially sensitive).
Positive control
Tonsil
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
Neuroscience >> Neurology process >> Neurogenesis
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAX5 antibody (ab15164)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab15164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr
Application notes
Immunohistology (Formalin/paraffin): 1/50 for 30 min at RT.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in disease
Note=A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Sequence similarities
Contains 1 paired domain.
Developmental stage
Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
Post-translational
modifications
O-glycosylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02548
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
see all
Database links
- Entrez Gene: 5079 Human
- Entrez Gene: 18507 Mouse
- Omim: 167414 Human
- SwissProt: Q02548 Human
- SwissProt: Q02650 Mouse
- Unigene: 654464 Human
- Unigene: 439659 Mouse
Anti-PAX5 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAX5 antibody (ab15164)
ab15164 staining PAX5 in human tonsil by Immunohistochemistry (FFPE-sections).
References for Anti-PAX5 antibody (ab15164)
ab15164 has not yet been referenced specifically in any publications.
Publishing research using ab15164? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"