PAX5 antibody (ab2935)

Product Name 

PAX5 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to PAX5

Immunogen 

Synthetic peptide: DLEKNYPTPRTSR, corresponding to N terminal amino acids 2-14 of Human PAX5.
BLAST 'DLEKNYPTPRTSR-C' with or

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 51 kDa in U937 cells (predicted molecular weight: 42 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

U937 lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX

Relevance 

The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

PAX5 expression is not only continuously required for B cell lineage commitment during early B cell development but also for B lineage maintenance. PAX5 is found in most cases of mature and precursor B cell non-Hodgkin lymphomas/leukemias. Pax-5 is not detected in Multiple myeloma and solitary plasmacytoma cell lymphomas do express Pax5 except for those with terminal B cell differentiation. T cell neoplasms do not stain with anti-Pax5; however there is a strong
association with CD20 expression.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Tris saline, 0.02% Na azide, pH7.3 with 0.5% BSA

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Principal Names – PAX5; BSAP; paired box gene 5 (B-cell lineage specific activator protein); B-cell lineage specific activator protein; paired box homeotic gene 5 (B-cell lineage specific activator protein)
Official Gene Symbol - PAX5
GenBank Accession Number – NP_057953
LocusLink ID - 5079 (human)
Gene Ontology terms - transcription activating factor; humoral immune response; histogenesis and organogenesis; development; oncogenesis; transcription from Pol II promoter

See below for PAX5 antibody images, references, products related to ab2935 and other tools.

PAX5 antibody images:

Customer reviews (feedback) regarding PAX5 antibody

Customer FAQs regarding PAX5 antibody

Protocols for PAX5 antibody

Price and availability of products related to PAX5 antibody

PAX5 antibody - related products: