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Overview
Product name
Anti-PAX5 antibody
See all PAX5 products (15) ...
Description
Goat polyclonal to PAX5
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: DLEKNYPTPRTSR, corresponding to N terminal amino acids 2-14 of Human PAX5.
DLEKNYPTPR TSR-C
Positive control
U937 lysate
General notes
Principal Names – PAX5; BSAP; paired box gene 5 (B-cell lineage specific activator protein); B-cell lineage specific activator protein; paired box homeotic gene 5 (B-cell lineage specific activator protein)
Official Gene Symbol - PAX5
GenBank Accession Number – NP_057953
LocusLink ID - 5079 (human)
Gene Ontology terms - transcription activating factor; humoral immune response; histogenesis and organogenesis; development; oncogenesis; transcription from Pol II promoter
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
Neuroscience >> Neurology process >> Neurogenesis
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Western blot - PAX5 antibody (ab2935)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 51 kDa in U937 cells (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.
Involvement in disease
Note=A chromosomal aberration involving PAX5 is a cause of acute lymphoblastic leukemia. Translocation t(9;18)(p13;q11.2) with ZNF521. Translocation t(9;3)(p13;p14.1) with FOXP1. Translocation t(9;12)(p13;p13) with ETV6.
Sequence similarities
Contains 1 paired domain.
Developmental stage
Expressed at early B-cell differentiation, in the developing CNS and in adult testis.
Post-translational
modifications
O-glycosylated.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ02548
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- B cell lineage specific activator antibody
- B cell lineage specific activator protein antibody
- B cell specific activator protein antibody
see all
Database links
- Entrez Gene: 5079 Human
- Omim: 167414 Human
- SwissProt: Q02548 Human
- Unigene: 654464 Human
Anti-PAX5 antibody images:
Western blot - PAX5 antibody (ab2935)
Predicted band size : 42 kDa
ab2935 staining (3µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
References for Anti-PAX5 antibody (ab2935)
ab2935 has not yet been referenced specifically in any publications.
Publishing research using ab2935? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"