Anti-PAX6 antibody [AD2.38] (ab78545)
- Product nameAnti-PAX6 antibody [AD2.38]See all PAX6 primary antibodies ...
- DescriptionMouse monoclonal [AD2.38] to PAX6
- SpecificityThis antibody recognizes both products of the two major alternatively spliced forms.
- Tested applicationsIHC-Fr, Sandwich ELISA, IHC-P, ICC/IF, IHC-FoFr more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken
Recombinant full length protein Human PAX6
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Monoclonal
- Clone numberAD2.38
Our Abpromise guarantee covers the use of ab78545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||IHC-Fr: Use at an assay dependent concentration.|
|Sandwich ELISA||sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PAX6 (ab82510). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to PAX6 (ab82510) as Detection.|
|IHC-P||IHC-P: Use at an assay dependent dilution.|
|ICC/IF||ICC/IF: Use at an assay dependent dilution.|
|IHC-FoFr||IHC-FoFr: Use at an assay dependent dilution.|
- FunctionTranscription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
- Tissue specificityFetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
- Involvement in diseaseDefects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
- Sequence similaritiesBelongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.
- Developmental stageExpressed in the developing eye and brain.
modificationsUbiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
- Cellular localizationNucleus.
- AN 2 antibodyAN antibodyAN2 antibody
- Aniridia type II protein antibodyD11S812E antibodyMGC17209 antibodyMGDA antibodyOculorhombin antibodyPaired box 6 antibodyPaired box gene 6 (aniridia keratitis) antibodyPaired Box Gene 6 antibodyPaired box homeotic gene 6 antibodyPaired box protein Pax-6 antibodyPaired box protein Pax6 antibodyPAX 6 antibodyPAX6 antibodyPAX6_HUMAN antibodySey antibodyWAGR antibody
Anti-PAX6 antibody [AD2.38] images
Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.
Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by NO. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.
References for Anti-PAX6 antibody [AD2.38] (ab78545)
This product has been referenced in:
- Zoldan J et al. The influence of scaffold elasticity on germ layer specification of human embryonic stem cells. Biomaterials 32:9612-21 (2011). IHC-P ; Human . Read more (PubMed: 21963156) »
- Stevens HE et al. Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits. J Neurosci 30:5590-602 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20410112) »