Overview

  • Product nameAnti-PAX6 antibody [AD2.38]See all PAX6 primary antibodies ...
  • Description
    Mouse monoclonal [AD2.38] to PAX6
  • SpecificityThis antibody recognizes both products of the two major alternatively spliced forms.
  • Tested applicationsIHC-Fr, Sandwich ELISA, IHC-P, Flow Cyt, ICC/IF, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Recombinant full length protein Human PAX6

Properties

Applications

Our Abpromise guarantee covers the use of ab78545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PAX6 (ab82510). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to PAX6 (ab82510) as Detection.
IHC-P Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionTranscription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
  • Tissue specificityFetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
  • Involvement in diseaseDefects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
    Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
    Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
    Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
    Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
    Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
    Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
    Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
  • Sequence similaritiesBelongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 paired domain.
  • Developmental stageExpressed in the developing eye and brain.
  • Post-translational
    modifications
    Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AN 2 antibody
    • AN antibody
    • AN2 antibody
    • Aniridia type II protein antibody
    • D11S812E antibody
    • MGC17209 antibody
    • MGDA antibody
    • Oculorhombin antibody
    • Paired box 6 antibody
    • Paired box gene 6 (aniridia keratitis) antibody
    • Paired Box Gene 6 antibody
    • Paired box homeotic gene 6 antibody
    • Paired box protein Pax-6 antibody
    • Paired box protein Pax6 antibody
    • PAX 6 antibody
    • PAX6 antibody
    • PAX6_HUMAN antibody
    • Sey antibody
    • WAGR antibody
    see all

Anti-PAX6 antibody [AD2.38] images

  • Standard Curve for Pax6; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [AD2.38] to PAX6 (ab78545) at 5 ug/ml and Detector Antibody Rabbit polyclonal to PAX6 (ab82510) at 0.5 ug/ml.
  • Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.

    Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.

    See Abreview

  • Overlay histogram showing SH-SY5Y cells stained with ab78545 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78545, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Protocols

References for Anti-PAX6 antibody [AD2.38] (ab78545)

This product has been referenced in:
  • Zoldan J  et al. The influence of scaffold elasticity on germ layer specification of human embryonic stem cells. Biomaterials 32:9612-21 (2011). IHC-P ; Human . Read more (PubMed: 21963156) »
  • Stevens HE  et al. Fgfr2 is required for the development of the medial prefrontal cortex and its connections with limbic circuits. J Neurosci 30:5590-602 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20410112) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E15.5 brain)
Specification E15.5 brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Nov 05 2012

Thank you for taking the time to complete our questionnaire.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your pu...

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I am CC'ing your lab manager on this reply as requested.

If you could provide the order number or PO number that was used to purchase ab78545, then I will go ahead and refund the cost of the antibody to you.<...

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Thank you for providing that image.

The difference in staining between the Pax6 and Tbr2 makes me think that protocol tips would not be effective in resolving this staining issue. Therefore I would like to either replace or refund the antibody...

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Thank you for calling Abcam earlier today.

I was wandering if you would be able to send an image of the staining that you are getting when you stain you mouse tissue with ab78545.

I would just like to assure you that the antibody is c...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab82510with the order number 1010290. To check the status of the orde...

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Thank you for contacting Abcam regarding ab78545. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free positive control ab29475 to test. To check the sta...

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Thank you for your reply.   I have contacted the lab to ask them to confirm what immunogen was used for ab78545. They confirmed that they used the N-terminal 206aa fragment as the immunogen.   Sorry for the confusion and I will have the datasheets upda...

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