• Product nameAnti-PAX6 antibody [AD2.38]
    See all PAX6 primary antibodies
  • Description
    Mouse monoclonal [AD2.38] to PAX6
  • SpecificityThis antibody recognizes both products of the two major alternatively spliced forms. In our hands this product does not detect a band of interest in Western Blot.
  • Tested applicationsSuitable for: IHC-Fr, Sandwich ELISA, IHC-P, ICC/IF, IHC-FoFrmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken
  • Immunogen

    Recombinant full length protein corresponding to Human PAX6.

  • Positive control
    • IHC-P: FFPE Human pancreas normal.



Our Abpromise guarantee covers the use of ab78545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PAX6 (ab82510). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to PAX6 (ab82510) as Detection.
IHC-P Use a concentration of 10 - 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionTranscription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.
    • Tissue specificityFetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.
    • Involvement in diseaseDefects in PAX6 are the cause of aniridia (AN) [MIM:106210]. A congenital, bilateral, panocular disorder characterized by complete absence of the iris or extreme iris hypoplasia. Aniridia is not just an isolated defect in iris development but it is associated with macular and optic nerve hypoplasia, cataract, corneal changes, nystagmus. Visual acuity is generally low but is unrelated to the degree of iris hypoplasia. Glaucoma is a secondary problem causing additional visual loss over time.
      Defects in PAX6 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly consists of a central corneal leukoma, absence of the posterior corneal stroma and Descemet membrane, and a variable degree of iris and lenticular attachments to the central aspect of the posterior cornea.
      Defects in PAX6 are a cause of foveal hypoplasia (FOVHYP) [MIM:136520]. Foveal hypoplasia can be isolated or associated with presenile cataract. Inheritance is autosomal dominant.
      Defects in PAX6 are a cause of keratitis hereditary (KERH) [MIM:148190]. An ocular disorder characterized by corneal opacification, recurrent stromal keratitis and vascularization.
      Defects in PAX6 are a cause of coloboma ocular (COLO) [MIM:120200]; also known as uveoretinal coloboma or coloboma of iris, choroid and retina. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Severe colobomatous malformations may cause as much as 10% of the childhood blindness. The clinical presentation of ocular coloboma is variable. Some individuals may present with minimal defects in the anterior iris leaf without other ocular defects. More complex malformations create a combination of iris, uveoretinal and/or optic nerve defects without or with microphthalmia or even anophthalmia.
      Defects in PAX6 are a cause of coloboma of optic nerve (COLON) [MIM:120430].
      Defects in PAX6 are a cause of bilateral optic nerve hypoplasia (BONH) [MIM:165550]; also known as bilateral optic nerve aplasia. A congenital anomaly in which the optic disc appears abnormally small. It may be an isolated finding or part of a spectrum of anatomic and functional abnormalities that includes partial or complete agenesis of the septum pellucidum, other midline brain defects, cerebral anomalies, pituitary dysfunction, and structural abnormalities of the pituitary.
      Defects in PAX6 are a cause of aniridia cerebellar ataxia and mental deficiency (ACAMD) [MIM:206700]; also known as Gillespie syndrome. A rare condition consisting of partial rudimentary iris, cerebellar impairment of the ability to perform coordinated voluntary movements, and mental retardation.
    • Sequence similaritiesBelongs to the paired homeobox family.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 paired domain.
    • Developmental stageExpressed in the developing eye and brain.
    • Post-translational
      Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • AN 2 antibody
      • AN antibody
      • AN2 antibody
      • Aniridia type II protein antibody
      • D11S812E antibody
      • FVH1 antibody
      • KIAA0552 antibody
      • Leucine zipper putative tumor suppressor 3 antibody
      • LZTS3 antibody
      • MGC17209 antibody
      • MGDA antibody
      • Oculorhombin antibody
      • Paired box 6 antibody
      • Paired box gene 6 (aniridia keratitis) antibody
      • Paired Box Gene 6 antibody
      • Paired box homeotic gene 6 antibody
      • Paired box protein Pax-6 antibody
      • Paired box protein Pax6 antibody
      • PAX 6 antibody
      • PAX6 antibody
      • PAX6_HUMAN antibody
      • ProSAP-interacting protein 1 antibody
      • PROSAPIP1 antibody
      • Sey antibody
      • WAGR antibody
      see all

    Anti-PAX6 antibody [AD2.38] images

    • IHC image of PAX6 staining in a formalin fixed, paraffin embedded normal human pancreas tissue section*performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78545, 20µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Immunohistochemical analysis of PFA-fixed frozen murine embryonic brain coronal sections, labelling PAX6 with ab78545 at a dilution of 1/100 incubated for 8 hours at 4°C in blocking buffer diluent. Permeabilization was with Triton X-100 and blocking was with 1% serum for 1 hour. Heat mediated antigen retrival was with 10mM sodium citrate buffer pH6.0 for 10 minutes at 650W in a microwave. The secondary was a goat Alexa Fluor® 488 at 1/700.

      See Abreview

    • IHC image of PAX6 staining in mouse e14 foetus formalin fixed paraffin embedded tissue section, with the use of Mouse on Mouse Polymer IHC Kit (Ab127055). The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab78545, 10µg/ml overnight at +4°C. The Mouse on Mouse HRP Polymer was incubated for 15 minutes at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    • Standard Curve for Pax6; dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [AD2.38] to PAX6 (ab78545) at 5 ug/ml and Detector Antibody Rabbit polyclonal to PAX6 (ab82510) at 0.5 ug/ml.
    • Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.

      Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.

      See Abreview


    References for Anti-PAX6 antibody [AD2.38] (ab78545)

    This product has been referenced in:
    • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). ICC/IF ; Human . Read more (PubMed: 26732624) »
    • Maury Y  et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol 33:89-96 (2015). Read more (PubMed: 25383599) »

    See all 8 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (embryonic mouse brain coronal tissue section)
    Permeabilization Yes - Triton-X 100
    Specification embryonic mouse brain coronal tissue section
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Fixative Paraformaldehyde

    Dr. Bhavin Shah

    Verified customer

    Submitted Oct 19 2015

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (brainstem)
    Permeabilization Yes - 0.1% triton
    Specification brainstem
    Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Oct 10 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (E15.5 brain)
    Specification E15.5 brain
    Fixative Paraformaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

    Abcam user community

    Verified customer

    Submitted Nov 05 2012

    Thank you for taking the time to complete our questionnaire.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    Reviewing this ca...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

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    Thank you for your reply.

    Your credit note ID is **.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your pu...

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    Thank you for your reply.

    I am CC'ing your lab manager on this reply as requested.

    If you could provide the order number or PO number that was used to purchase ab78545, then I will go ahead and refund the cost of the antibody to you.<...

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    Thank you for providing that image.

    The difference in staining between the Pax6 and Tbr2 makes me think that protocol tips would not be effective in resolving this staining issue. Therefore I would like to either replace or refund the antibody...

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    Thank you for calling Abcam earlier today.

    I was wandering if you would be able to send an image of the staining that you are getting when you stain you mouse tissue with ab78545.

    I would just like to assure you that the antibody is c...

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    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab82510with the order number 1010290. To check the status of the orde...

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