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Recombinant full length protein Human PAX6
Our Abpromise guarantee covers the use of ab78545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|Sandwich ELISA||Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to PAX6 (ab82510). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to PAX6 (ab82510) as Detection.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration.|
Immunohistochemical analysis of PFA-fixed frozen murine embryonic brain coronal sections, labelling PAX6 with ab78545 at a dilution of 1/100 incubated for 8 hours at 4°C in blocking buffer diluent. Permeabilization was with Triton X-100 and blocking was with 1% serum for 1 hour. Heat mediated antigen retrival was with 10mM sodium citrate buffer pH6.0 for 10 minutes at 650W in a microwave. The secondary was a goat Alexa Fluor® 488 at 1/700.
IHC image of PAX6 staining in mouse e14 foetus formalin fixed paraffin embedded tissue section, with the use of Mouse on Mouse Polymer IHC Kit (Ab127055). The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab78545, 10µg/ml overnight at +4°C. The Mouse on Mouse HRP Polymer was incubated for 15 minutes at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of mouse brain tissue, staining PAX6 with ab78545.
Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBST) for 12 hours at 4°C. An AlexaFluor®568-conjugated goat anti-mouse polyclonal IgG (1/1000) was used as the secondary antibody.
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