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Product name
Anti-PAX7 antibody
See all PAX7 products (10) ...
Description
Rabbit polyclonal to PAX7
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human PAX7.
(Peptide available as ab375 76.)
Positive control
ab34360 was positive in the following Whole Cell Lysates: HeLa (Human epithelial carcinoma cell line), Jurkat (Human T cell lymphoblast-like cell line), A431 (Human epithelial carcinoma cell line), HEK 293 (Human embryonic kidney cell line), MCF-7 (Human breast adenocarcinoma cell line), U2OS (Human osteosarcoma cell line).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Organogenesis >> Skeletal development >> Muscle
Developmental Biology >> Lineage specification >> Ectoderm
Stem Cells >> Mesenchymal Stem Cells >> Myogenesis
Stem Cells >> Lineage Markers >> Ectoderm
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> PAX
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Western blot - PAX7 antibody (ab34360)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
ICC/IF: Use at an assay dependent dilution. PubMed: 20723755
Target
Function
Probable transcription factor. May have a role in myogenesis.
Involvement in disease
Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2) [MIM:268220]. It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. Note=A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator.
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Contains 1 paired domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP23759
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ37460 antibody
- HUP1 antibody
- OTTHUMP00000002534 antibody
see all
Database links
- Entrez Gene: 5081 Human
- Entrez Gene: 18509 Mouse
- Entrez Gene: 500574 Rat
- Omim: 167410 Human
- SwissProt: P23759 Human
- SwissProt: P47239 Mouse
- Unigene: 113253 Human
- Unigene: 218760 Mouse
Anti-PAX7 antibody images:
Western blot - PAX7 antibody (ab34360)
All lanes : Anti-PAX7 antibody (ab34360) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 57 kDa
Observed band size : 60 kDa (why is the actual band size different from the predicted?)
References for Anti-PAX7 antibody (ab34360)
This product has been referenced in:
- Guo BS et al. Electrical stimulation influences satellite cell proliferation and apoptosis in unloading-induced muscle atrophy in mice. PLoS One 7:e30348 (2012). ICC/IF; Mouse.Read more (PubMed: 22253929) »
- Han XH et al. A WNT/{beta}-catenin signaling activator, R-spondin, plays positive regulatory roles during skeletal myogenesis. J Biol Chem : (2011). WB; Mouse.Read more (PubMed: 21252233) »
See all 4 publications for this product
Publishing research using ab34360? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"