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Anti-PBEF antibody [1D3A12] (ab66887)

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Overview

Product name

Anti-PBEF antibody [1D3A12]
See all PBEF products (12) ...

Description

Mouse monoclonal [1D3A12] to PBEF

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Pig, Carp, Pufferfish (Fugu)

Immunogen

Recombinant His tagged fragment corresponding to amino acids 338-479 of Human PBEF

SKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIEN IAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDP VADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDL LHTVFKNGKVTKSYSFDEIRKN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

truncated PBEF-His recombinant protein and transfected GFP-PBEF Cos7 cells lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

1D3A12

Isotype

IgG1

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Immunology >> Innate Immunity >> Cytokines >> Other
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Hypoxia >> Hypoxia-Regulated

  • Western blot - PBEF antibody [1D3A12] (ab66887)Western blot - PBEF antibody [1D3A12] (ab66887) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/2000. Pred...Read more →

    WB: 1/500 - 1/2000. Predicted molecular weight: 56 kDa.

  • ELISA

     ELISA: 1/10000.

    ELISA: 1/10000.

Target

Function

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.

Tissue specificity

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.

Sequence similarities

Belongs to the NAPRTase family.

Cellular localization

Cytoplasm.

Target information above from: UniProt accessionP43490 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AI480535 antibody
  • 1110035O14Rik antibody
  • AI314458 antibody
  • DKFZP666B131 antibody
  • EC 2.4.2.12 antibody
  • MGC117256 antibody
  • NAmPRTase antibody
  • NAMPT antibody
  • NAMPT_HUMAN antibody
  • Nicotinamide phosphoribosyltransferase antibody
  • PBEF 1 antibody
  • PBEF antibody
  • PBEF1 antibody
  • Pre B cell colony enhancing factor 1 antibody
  • Pre B cell colony enhancing factor antibody
  • Pre B cell enhancing factor antibody
  • Pre-B cell-enhancing factor antibody
  • Pre-B-cell colony-enhancing factor 1 antibody
  • VF antibody
  • Visfatin antibody
see all

Database links

see all

Anti-PBEF antibody [1D3A12] images:

  Western blot - PBEF antibody [1D3A12] (ab66887)

Western blot - PBEF antibody [1D3A12] (ab66887)

All lanes : Anti-PBEF antibody [1D3A12] (ab66887) at 1/500 dilution

Lane 1 : Truncated PBEF-His recombinant protein (aa338-479;16kDa)
Lane 2 : Whole cell lysate from COS-7 cell line transfected with full lenght GFP-PBEF (27kDa+56kDa; aa1-491)


Predicted band size : 56 kDa

References for Anti-PBEF antibody [1D3A12] (ab66887)

ab66887 has not yet been referenced specifically in any publications.

Publishing research using ab66887? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"