![Western blot - PBEF antibody [4E11C9] (ab59106) image](#ab59106.jpg)
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Product name
Anti-PBEF antibody [4E11C9]
See all PBEF products (12) ...
Description
Mouse monoclonal [4E11C9] to PBEF
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
Ni-NTA purified truncated recombinant PBEF expressed in E. coli strain BL21 (DE3)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
4E11C9
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Immunology >> Innate Immunity >> Cytokines >> Other
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
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Western blot - PBEF antibody [4E11C9] (ab59106)
![Western blot - PBEF antibody [4E11C9] (ab59106) image](/ps/datasheet/Images/59/ab59106/ab59106.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab59106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/200 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
Target
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
Sequence similarities
Belongs to the NAPRTase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP43490
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI480535 antibody
- 1110035O14Rik antibody
- AI314458 antibody
see all
Database links
- Entrez Gene: 10135 Human
- Omim: 608764 Human
- SwissProt: P43490 Human
- Unigene: 489615 Human
Anti-PBEF antibody [4E11C9] images:
Western blot - PBEF antibody [4E11C9] (ab59106)
Lane 1 : marker
Lane 2 : Anti-PBEF antibody [4E11C9] (ab59106) at 1/200 dilution
Lane 1 : As above
Lane 2 : truncated PBEF recombinant protein
Predicted band size : 56 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
References for Anti-PBEF antibody [4E11C9] (ab59106)
ab59106 has not yet been referenced specifically in any publications.
Publishing research using ab59106? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"