![Western blot - PBEF antibody [EPR3392] (ab109210) image](#PBEF-Primary-antibodies-ab109210-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210) image](#PBEF-Primary-antibodies-ab109210-2.gif)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210) image](#PBEF-Primary-antibodies-ab109210-3.gif)
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Product name
Anti-PBEF antibody [EPR3392]
See all PBEF products (12) ...
Description
Rabbit monoclonal [EPR3392] to PBEF
Tested applications
WB, IHC-P, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues in Human PBEF.
Positive control
WB: Fetal liver, Human skeletal muscle, fetal kidney, and Human heart lysates IHC-P: Human kidney and liver tissues
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3392
Isotype
IgG1
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Insulin / Insulin-like
Immunology >> Innate Immunity >> Cytokines >> Other
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Hypoxia >> Hypoxia-Regulated
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
ICC
ICC: 1/50 - 1/100.ICC: 1/50 - 1/100.
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
Tissue specificity
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
Sequence similarities
Belongs to the NAPRTase family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP43490
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AI480535 antibody
- 1110035O14Rik antibody
- AI314458 antibody
see all
Database links
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Entrez Gene: 297508 Rat
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q3TLX1 Mouse
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q80Z29 Rat
see all
Anti-PBEF antibody [EPR3392] images:
Western blot - PBEF antibody [EPR3392] (ab109210)
![Western blot - PBEF antibody [EPR3392] (ab109210)](/ps/datasheet/images/109/ab109210/PBEF-Primary-antibodies-ab109210-1.jpg)
All lanes : Anti-PBEF antibody [EPR3392] (ab109210) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : Human skeletal muscle lysate
Lane 3 : Fetal kidney lysate
Lane 4 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size : 56 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210)](/ps/datasheet/images/109/ab109210/PBEF-Primary-antibodies-ab109210-2.gif)
ab109210, at 1/250 dilution, staining PBEF in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBEF antibody [EPR3392] (ab109210)](/ps/datasheet/images/109/ab109210/PBEF-Primary-antibodies-ab109210-3.gif)
ab109210, at 1/250 dilution, staining PBEF in paraffin-embedded Human liver tissue by Immunohistochemistry.
Western blot - Anti-PBEF antibody [EPR3392] (ab109210)
![Western blot - Anti-PBEF antibody [EPR3392] (ab109210)](/ps/datasheet/images/109/ab109210/PBEF-Primary-antibodies-ab109210-4.jpg)
All lanes : Anti-PBEF antibody [EPR3392] (ab109210) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate
Predicted band size : 56 kDa
Observed band size : 56 kDa
Additional bands at : 120 kDa,45 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBEF antibody [EPR3392] (ab109210)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBEF antibody [EPR3392] (ab109210)](/ps/datasheet/images/109/ab109210/PBEF-Primary-antibodies-ab109210-5.jpg)
ab109210 staining at 1/250 dilution staining PBEF in mouse liver tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
References for Anti-PBEF antibody [EPR3392] (ab109210)
ab109210 has not yet been referenced specifically in any publications.
Publishing research using ab109210? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"