Overview

  • Product nameAnti-PBK antibody [EP2520Y]
    See all PBK primary antibodies
  • Description
    Rabbit monoclonal [EP2520Y] to PBK
  • Tested applicationsWB, IP, ICC, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues from the C-terminus of human PBK

  • Positive control
    • Human tonsil. HeLa cell lysate. IF/ICC: PFA-fixed JEG-3 cell line.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab75987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000 - 1/500000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/30.
ICC 1/100 - 1/250.
Flow Cyt 1/1000.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

Target

  • FunctionPhosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
  • Tissue specificityExpressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated; in a cell-cycle dependent manner at mitosis.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancer/testis antigen 84 antibody
    • CT84 antibody
    • Epididymis luminal protein 164 antibody
    • FLJ14385 antibody
    • HEL164 antibody
    • Lymphokine activated killer T cell originated protein kinase antibody
    • Lymphokine-activated killer T-cell-originated protein kinase antibody
    • MAPKK like protein kinase antibody
    • MAPKK-like protein kinase antibody
    • Nori 3 antibody
    • Nori-3 antibody
    • Nori3 antibody
    • PBK antibody
    • PDZ binding kinase antibody
    • PDZ-binding kinase antibody
    • Serine/threonine protein kinase antibody
    • Spermatogenesis related protein kinase antibody
    • Spermatogenesis-related protein kinase antibody
    • SPK antibody
    • T LAK cell originated protein kinase antibody
    • T-LAK cell-originated protein kinase antibody
    • TOPK antibody
    • TOPK_HUMAN antibody
    see all

Anti-PBK antibody [EP2520Y] images

  • ab75987 stained JEG-3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75987 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Overlay histogram showing JEG3 cells stained with ab75987 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75987, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in JEG3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-PBK antibody [EP2520Y] (ab75987) at 1/500000 dilution + HeLa cell lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human tonsil using ab75987 at 1/100 dilution.

References for Anti-PBK antibody [EP2520Y] (ab75987)

This product has been referenced in:
  • Guo Z  et al. Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase. J Proteome Res 11:4847-62 (2012). Read more (PubMed: 22889411) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"