Overview

  • Product nameAnti-PBK antibody [EP2520Y]
    See all PBK primary antibodies
  • Description
    Rabbit monoclonal [EP2520Y] to PBK
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues from the C-terminus of human PBK

  • Positive control
    • Human tonsil. HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100000 - 1/500000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
IP 1/30.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended.

  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionPhosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
    • Tissue specificityExpressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Phosphorylated; in a cell-cycle dependent manner at mitosis.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancer/testis antigen 84 antibody
      • CT84 antibody
      • Epididymis luminal protein 164 antibody
      • FLJ14385 antibody
      • HEL164 antibody
      • Lymphokine activated killer T cell originated protein kinase antibody
      • Lymphokine-activated killer T-cell-originated protein kinase antibody
      • MAPKK like protein kinase antibody
      • MAPKK-like protein kinase antibody
      • Nori 3 antibody
      • Nori-3 antibody
      • Nori3 antibody
      • PBK antibody
      • PDZ binding kinase antibody
      • PDZ-binding kinase antibody
      • Serine/threonine protein kinase antibody
      • Spermatogenesis related protein kinase antibody
      • Spermatogenesis-related protein kinase antibody
      • SPK antibody
      • T LAK cell originated protein kinase antibody
      • T-LAK cell-originated protein kinase antibody
      • TOPK antibody
      • TOPK_HUMAN antibody
      see all

    Anti-PBK antibody [EP2520Y] images



    • Predicted band size : 36 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: PBK knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Human tonsil tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab75987 observed at 40 kDa. Red - loading control, ab7291, observed at 52 kDa.

      ab75987 was shown to recognize PBK when PBK knockout samples were used, along with additional cross-reactive bands. Wild-type and PBK knockout samples were subjected to SDS-PAGE. ab75987 diluted to 1/100,000 and ab7291 (loading control to alpha Tubulin) diluted to 1/10,000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

    • Anti-PBK antibody [EP2520Y] (ab75987) at 1/500000 dilution + HeLa cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 36 kDa
      Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human tonsil using ab75987 at 1/100 dilution.

    References for Anti-PBK antibody [EP2520Y] (ab75987)

    This product has been referenced in:
    • Guo Z  et al. Proteomics strategy to identify substrates of LNX, a PDZ domain-containing E3 ubiquitin ligase. J Proteome Res 11:4847-62 (2012). Read more (PubMed: 22889411) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"