Anti-PBK antibody [EP2520Y] (ab75987)

Overview

• Product nameAnti-PBK antibody [EP2520Y]See all PBK primary antibodies ...
• Description
  Rabbit monoclonal [EP2520Y] to PBK
• Tested applicationsWB, IP, ICC, Flow Cyt, IHC-P more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues from the C-terminus of human PBK

• Positive controlHuman tonsil. HeLa cell lysate.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP2520Y
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • p53 pathway

  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • DNA Damage Response
  • p53

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • p53

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Cytokinesis

Applications

Target

• FunctionPhosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
• Tissue specificityExpressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
• Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily.
  Contains 1 protein kinase domain.
• Post-translational
  modificationsPhosphorylated; in a cell-cycle dependent manner at mitosis.

Target information above from: UniProt accession Q96KB5 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 55872 Human
  • Omim: 611210 Human
  • SwissProt: Q96KB5 Human
  • Unigene: 104741 Human

• Alternative names
  Cancer/testis antigen 84 antibodyCT84 antibodyFLJ14385 antibody

  Lymphokine activated killer T cell originated protein kinase antibodyLymphokine-activated killer T-cell-originated protein kinase antibodyMAPKK like protein kinase antibodyMAPKK-like protein kinase antibodyNori 3 antibodyNori-3 antibodyNori3 antibodyPBK antibodyPDZ binding kinase antibodyPDZ-binding kinase antibodySerine/threonine protein kinase antibodySpermatogenesis related protein kinase antibodySpermatogenesis-related protein kinase antibodySPK antibodyT LAK cell originated protein kinase antibodyT-LAK cell-originated protein kinase antibodyTOPK antibodyTOPK_HUMAN antibody

  see all

Anti-PBK antibody [EP2520Y] images

• 

  Western blot - PBK antibody [EP2520Y] (ab75987)
  Anti-PBK antibody [EP2520Y] (ab75987) at 1/500000 dilution + HeLa cell lysate at 10 µg
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 36 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBK antibody [EP2520Y] (ab75987)
  Immunohistochemical analysis of paraffin-embedded human tonsil using ab75987 at 1/100 dilution.