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- Proteins and Peptides
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Product name
Anti-PBP antibody
See all PBP products (8) ...
Description
Goat polyclonal to PBP
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
Synthetic peptide: DYVPKLYEQLSGK, corresponding to C terminal amino acids 174-186 of Human PBP.
C-DYVPKLYEQLSGK
Positive control
A549, HepG2, HEK293 and human liver lysate.
General notes
GenBank Accession Number – NP_002558.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Neuroscience >> Neurotransmitter >> Neuropeptides >> More Neuropeptides
Neuroscience >> Neurotransmitter >> Acetylcholine
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Cell Biology >> Cell Cycle >> Kinases/Phosphatases >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 20-22 kDa (predicted molecular weight: 22 kDa).
Target
Function
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Sequence similarities
Belongs to the phosphatidylethanolamine-binding protein family.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionP30086
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- HCNP antibody
- HCNPpp antibody
- Hippocampal cholinergic neurostimulating peptide antibody
see all
Database links
- Entrez Gene: 431786 Cow
- Entrez Gene: 477501 Dog
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P13696 Cow
- SwissProt: Q3YIX4 Dog
see all
Anti-PBP antibody images:
Western blot - PBP antibody (ab2634)
Predicted band size : 22 kDa
ab2634 staining (0.01 µg/ml) of A549 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBP antibody (ab2634)
ab2634 (1.5 µg/ml) staining PBP in paraffin-embedded Human Cortex following steamed antigen retrieval with citrate buffer at pH 6.
References for Anti-PBP antibody (ab2634)
This product has been referenced in:
- Fougner SL et al. Low levels of raf kinase inhibitory protein in growth hormone-secreting pituitary adenomas correlate with poor response to octreotide treatment. J Clin Endocrinol Metab 93:1211-6 (2008). WB; Human.Read more (PubMed: 18230656) »
- Chen Q et al. Identification and characterization of PEBP as a calpain substrate. J Neurochem 99:1133-41 (2006).Read more (PubMed: 17018026) »
See all 2 publications for this product
Publishing research using ab2634? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"