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Overview

  • Product namePBP protein (Tagged-His Tag)See all PBP proteins and peptides ...
  • Protein descriptionRecombinant full length protein, corresponding to amino acids 1-187 of Human PBP with N terminal His tag; Predicted MWt 22 kDa (UniProt P30086).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 106 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesMPVDLSKWSGPLSLQEVDEQPQHPLHVTYAGAAVDELGKV LTPTQVKN RPTSISWDGLDSGKLYTLVLTDPDAPSRKD PKYREWHHFLVVNMKGND ISSGTVLSDYVGSGPPKGTG LHRYVWLVYEQDRPLKCDEPILSNRSGD HRGKFKVASF RKKYELRAPVAGTCYQAEWDDYVPKLYEQLSGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91871 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        HCNPHCNPppHippocampal cholinergic neurostimulating peptide
        Neuropolypeptide h3PEBPPEBP 1PEBP-1PEBP1PEBP1_HUMANPhosphatidylethanolamine binding proteinPhosphatidylethanolamine binding protein 1Prostatic binding proteinProstatic-binding proteinR kipRaf kinase inhibitor proteinRaf kinase inhibitory proteinRKIP
      see all
  • FunctionBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
    HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
  • Sequence similaritiesBelongs to the phosphatidylethanolamine-binding protein family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P30086 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    PBP protein (Tagged-His Tag) images

    • SDS-PAGE - PBP protein (Tagged-His Tag) (ab91871)
      SDS-PAGE - PBP protein (Tagged-His Tag) (ab91871)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for PBP protein (Tagged-His Tag) (ab91871)

    ab91871 has not yet been referenced specifically in any publications.

    Publishing research using ab91871 ? Please let us know so that we can cite the reference in this datasheet

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