Anti-PBR antibody [EPR5384] (ab109497)
- Product nameAnti-PBR antibody [EPR5384]See all PBR primary antibodies ...
- DescriptionRabbit monoclonal [EPR5384] to PBR
- Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt more details
- Species reactivityReacts with: Mouse, Human
Does not react withRat
A synthetic peptide corresponding to residues at the C-terminus of Human PBR.
- Positive controlU-87MG, 293T, RAW264.7, and NIH3T3 cell lysates; Human colon tissue; A431 cells.
- General notesProduced under U.S. Patent No. 5,675,063.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR5384
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab109497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/10000 - 1/50000. Predicted molecular weight: 19 kDa.Can be blocked with PBR peptide (ab170987).|
|IP||IP: 1/10 - 1/100.|
|IHC-P||IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC||ICC: 1/50 - 1/100.|
|Flow Cyt||Flow Cyt: 1/50.|
- FunctionResponsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
- Tissue specificityFound in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
- Sequence similaritiesBelongs to the TspO/BZRP family.
- Cellular localizationMitochondrion membrane.
- Benzodiazapine receptor (peripheral) antibodyBenzodiazepine peripheral binding site antibodyBPBS antibody
- BZRP antibodyDBI antibodyIBP antibodyIsoquinoline carboxamide-binding protein antibodyMBR antibodymDRC antibodyMitochondrial benzodiazepine receptor antibodyPBR antibodyPBS antibodyPeripheral benzodiazepine receptor antibodyPeripheral benzodiazepine receptor-related protein antibodyPeripheral type benzodiazepine receptor antibodyPeripheral-type benzodiazepine receptor antibodypk18 antibodyPKBS antibodyPTBR antibodyPtbzr antibodyPTBZR02 antibodyRATPTBZR02 antibodytranslocator protein (18kDa) antibodyTranslocator protein antibodyTSPO antibodyTspo1 antibodyTSPOA_HUMAN antibody
Anti-PBR antibody [EPR5384] images
All lanes : Anti-PBR antibody [EPR5384] (ab109497) at 1/10000 dilution
Lane 1 : U-87 MG cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : RAW264.7 cell lysate
Lane 5 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size : 19 kDa
ab109497 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
ab109497 at 1/50 dilution staining PBR in A431 cells
Overlay histogram showing HepG2 cells stained with ab109497 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109497, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-PBR antibody [EPR5384] (ab109497)
ab109497 has not yet been referenced specifically in any publications.