Anti-PBR antibody [EPR5384] (ab109497)

Overview

• Product nameAnti-PBR antibody [EPR5384]See all PBR primary antibodies ...
• Description
  Rabbit monoclonal [EPR5384] to PBR
• Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  A synthetic peptide corresponding to residues at the C-terminus of Human PBR.

• Positive controlU-87MG, 293T, RAW264.7, and NIH3T3 cell lysates; Human colon tissue; A431 cells.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR5384
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Cholesterol Metabolism

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Signal Transduction
  • Signaling Pathway
  • Calcium Signaling
  • Calcium Channels

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cardiovascular
  • Lipids / Lipoproteins
  • Lipid Metabolism
  • Cholesterol Metabolism

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionResponsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells.
• Tissue specificityFound in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.
• Sequence similaritiesBelongs to the TspO/BZRP family.
• Cellular localizationMitochondrion membrane.

Target information above from: UniProt accession P30536 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 706 Human
  • Entrez Gene: 12257 Mouse
  • Omim: 109610 Human
  • SwissProt: B1AH88 Human
  • SwissProt: P30536 Human
  • SwissProt: P50637 Mouse
  • Unigene: 202 Human
  • Unigene: 1508 Mouse

• Alternative names
  Benzodiazapine receptor (peripheral) antibodyBenzodiazepine peripheral binding site antibodyBPBS antibody

  BZRP antibodyDBI antibodyIBP antibodyIsoquinoline carboxamide-binding protein antibodyMBR antibodymDRC antibodyMitochondrial benzodiazepine receptor antibodyPBR antibodyPBS antibodyPeripheral benzodiazepine receptor antibodyPeripheral benzodiazepine receptor-related protein antibodyPeripheral type benzodiazepine receptor antibodyPeripheral-type benzodiazepine receptor antibodypk18 antibodyPKBS antibodyPTBR antibodyPtbzr antibodyPTBZR02 antibodyRATPTBZR02 antibodytranslocator protein (18kDa) antibodyTranslocator protein antibodyTSPO antibodyTspo1 antibodyTSPOA_HUMAN antibody

  see all

Anti-PBR antibody [EPR5384] images

• 

  Western blot - PBR antibody [EPR5384] (ab109497)
  All lanes : Anti-PBR antibody [EPR5384] (ab109497) at 1/10000 dilution
  
  Lane 1 : U-87 MG cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : A431 cell lysate
  Lane 4 : RAW264.7 cell lysate
  Lane 5 : NIH3T3 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 19 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PBR antibody [EPR5384] (ab109497)
  ab109497 at 1/100 dilution staining PBR in paraffin embedded Human colon tissue.
• 

  Immunocytochemistry/ Immunofluorescence - PBR antibody [EPR5384] (ab109497)
  ab109497 at 1/50 dilution staining PBR in A431 cells
• 

  Flow Cytometry - Anti-PBR antibody [EPR5384] (ab109497)
  Overlay histogram showing HepG2 cells stained with ab109497 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109497, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.