PC1/3 antibody - Aminoterminal end (ab28551)

Product Name 

PC1/3 antibody - Aminoterminal end

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PC1/3 - Aminoterminal end

Immunogen 

Synthetic peptide (Human)(Peptide available as a b41400.)

Reacts with 
(species key)

Hu

Specificity 

ab28551 recognizes subtilisin-like serine protease PC1.

We have a range of domain specific antibodies for this target. For a full list please see all PC1/3 antibodies

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: use 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 84 kDa. Glycosylation and other post-translational modifications make the pre-pro PC1 run at 94 kDa, the mature PC1 at 87 and 83 kDa and carboxy-processed PC1 at 66 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

PC1 is expressed at low levels in most tissues, but found in highest concentration in the brain, the anterior pituitary, pancreatic islet cells, thyroid, parathyroid and gut.

Cellular localization 

Golgi Apparatus and Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases

Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

This antibody has been peptide-affinity purified.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

A literature alias is PC3, and PC1 is often referred to as PC1/3.

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