Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Growth Factors/Hormones >> Hormones
Overview
Product name
Anti-PC1/3 antibody - Aminoterminal end
See all PC1/3 products (7) ...
Description
Rabbit polyclonal to PC1/3 - Aminoterminal end
Specificity
ab28551 recognizes subtilisin-like serine protease PC1.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide (Human)(Peptide available as ab41400.)
Positive control
PC1 is expressed at low levels in most tissues, but found in highest concentration in the brain, the anterior pituitary, pancreatic islet cells, thyroid, parathyroid and gut.
General notes
A literature alias is PC3, and PC1 is often referred to as PC1/3.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Growth Factors/Hormones >> Hormones
-
Western blot - PC1/3 antibody - Aminoterminal end (ab28551)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: use 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 84 kDa. Glycosylation and other post-translational modifications make the pre-pro PC1 run at 94 kDa, the mature PC1 at 87 and 83 kDa and carboxy-processed PC1 at 66 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
Involvement in disease
Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.
Sequence similarities
Belongs to the peptidase S8 family. Furin subfamily.
Cellular localization
Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.
Target information above from: UniProt accessionP29120
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ATT1 antibody
- ATT1 antibody
- BDP antibody
see all
Database links
- Entrez Gene: 5122 Human
- Omim: 162150 Human
- SwissProt: P29120 Human
- Unigene: 78977 Human
Anti-PC1/3 antibody - Aminoterminal end images:
Western blot - PC1/3 antibody - Aminoterminal end (ab28551)
All lanes : Anti-PC1/3 antibody - Aminoterminal end (ab28551) at 1/1000 dilution
Lane 1 : SF treated Hs1.Tes Lysate
Lane 2 : TPA Hs1.Tes Lysate
Lane 3 : IL1-beta Hs1.Tes Lysate
Lane 4 : TNF-alpha Hs1.Tes Lysate
Lane 5 : SF treated ST Lysate
Lane 6 : TPA treated SF Lysate
Lane 7 : IL1-beta ST Lysate
Lysates/proteins at 10 µl per lane.
Predicted band size : 84 kDa
Observed band size : 68,96 kDa (why is the actual band size different from the predicted?)
Additional bands at : 108 kDa (possible dimer).
Protocols
References for Anti-PC1/3 antibody - Aminoterminal end (ab28551)
ab28551 has not yet been referenced specifically in any publications.
Publishing research using ab28551? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"