You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-PC1/3 antibody (ab11786)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-PC1/3 antibody
See all PC1/3 products (7) ...

Description

Rabbit polyclonal to PC1/3

Specificity

This antibody recognises the peptide enzyme designated proprotein convertase 1 and also known as PC1/3, NEC1 and neuroendocrine convertase 1. This enzyme is involved in the processing of hormone (and other protein) precursors including renin, somatostatin and insulin. It is found in secretory granules. The PC1/3 antibody recognises the endogenous protein in rat and human pancreatic islets and in the RIN cell line. We have also seen reactivity in brain extracts though this is faint unless glycoproteins are enriched by affinity-purification on concanavalin A. The protein recognized by this antibody has 3 forms. These proteins are a 93 kDa which is the full length glycosylated protein (i.e. includes the signal peptide), a 91 kDa which is the glycosylated protein less the signal peptide, and a 66 kDa which is the active protein. In WB, this antibody only picks up a 66 kDa band. The 93 kDa protein is unlikely to occur in nature due to the removal of the signal peptide during co-translational insertion into the endoplasmic reticulum, and the 91 kDa protein may occur briefly during transport from the ER to the Golgi, where the propeptide is removed to yield the active 66 kDa protein. Since these processing steps all occur at the N-terminus and the antibody is directed at the C-terminus, the antibody would be expected to recognize all three forms.

Tested applications

WBmore details

Cross reactivity

Reacts with

Rat, Human, Monkey

Immunogen

Recombinant full length protein (Human)

Positive control

pancreatic islets lysate, RIN cell lysate

General notes

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Signal Transduction >> Growth Factors/Hormones >> Hormones

Applications

Show applications key

Our Abpromise guarantee covers the use of ab11786 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • WB

     

Application notes

WB: 1/500 - 1/1000. Predicted molecular weight: See specificity notes


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

Involvement in disease

Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

Sequence similarities

Belongs to the peptidase S8 family. Furin subfamily.

Cellular localization

Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.

Target information above from: UniProt accessionP29120 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • ATT1 antibody
  • BDP antibody
  • Furin homolog antibody
  • NEC 1 antibody
  • NEC1 antibody
  • NEC1_HUMAN antibody
  • Neuroendocrine convertase 1 antibody
  • PC1 antibody
  • PC3 antibody
  • Pcsk1 antibody
  • Phpp1 antibody
  • Prohormone convertase 1 antibody
  • Prohormone convertase 1/3 antibody
  • Prohormone convertase 3 antibody
  • Prohormone convertase antibody
  • Propeptide processing protease antibody
  • Proprotein convertase 1 antibody
  • Proprotein convertase subtilisin/kexin type 1 antibody
  • Protein convertase subtilisin/kexin type I antibody
  • SPC3 antibody
see all

Database links

References for Anti-PC1/3 antibody (ab11786)

ab11786 has not yet been referenced specifically in any publications.

Publishing research using ab11786? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"